Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A009
DOI: https://doi.org/10.24326/ICDSUPL1.A009
Published online: 26 April 2022
ICDSUPL, 1, A009 (2022)
Can fermented whey modulate AFB1- and OTA-induced toxicity in vivo?
Massimo Frangiamone1*, Alexander Yemelin2, Alessandra Cimbalo1, Luis Antelo3, Eckhard Thines3, Lara Manyes Font1
1 Laboratory of Food Chemistry and Toxicology, Faculty of Pharmacy, Universitat de València, Vicent Andrés Estellés s/n, Burjassot, Spain
2 Institut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), Erwin-Schrödinger. 56, D-67663 Kaiserslautern, Germany
3 Mikrobiology and Biotechnology, Hanns-Dieter-Hüsch-Weg 17, 55128 Mainz, Germany
* Corresponding author: massimo2.frangiamone@uv.es
Abstract
Due to the globalization, mycotoxins have been considered a major risk to human health being the main contaminants of foodstuffs. Among them, aflatoxin B1 (AFB1) and ochratoxin A (OTA) are the most toxic and studied. Dietary exposure to AFB1 and OTA has been associated with several human and animal diseases, including hepatotoxicity and nephrotoxicity. In this context, carbamoyl phosphate synthetase 1 (CPS1) and kidney injury molecule 1 (KIM-1) are typical biomarkers of liver and kidney damage, respectively. Fermented Whey (FW), derived from milk whey fermentation, contains several bioactive compounds (such as organic and phenolic acids) that show antifungal, antioxidant and immunomodulatory proprieties. Thus, FW may be considered a plausible candidate against AFB1- and OTA-toxicity. In view of this, a 28 days in vivo study was performed in 70 rats divided in 7 groups (10 rats for each, 5 males and 5 females) fed with: 1) control diet; 2) AFB1 (5±0.6 mg/kg); 3) OTA (10.2±1.1 mg/kg); 4) AFB1 and OTA (8.8±1.5 and 10.9 mg/kg respectively); 5) FW (1%) and AFB1 (6.1±1.4 mg/kg); 6) FW (1%) and OTA (6.1±0.3 mg/kg); 7) FW (1%) with AFB1 and OTA (8.4±0.3 mg/kg and 8.4±0.4 mg/kg respectively). RNA extraction will be performed from rat liver and kidney tissue and 3 biological replicates will be considered. The potential beneficial effect of FW against AFB1- and OTA-contaminated feed will be investigated by analyzing the alteration in gene expression of CPS1 (liver) and KIM-1 (kidney) from each experimental group. For this purpose, the relative and absolute quantification of these biomarkers is being carried out using qPCR and droplet digital PCR (ddPCR) techniques, respectively. The future results may contribute to a better understanding of FW activity against AFB1- and OTA-toxicity at molecular level in vivo.
This work was supported by Spanish Ministry of Science and Innovation (PID2019-108070RB-I00-ALI).
How to cite
M. Frangiamone, A. Yemelin, A. Cimbalo, L. Antelo, E. Thines, L. Manyes Font, 2022. Can fermented whey modulate AFB1- and OTA-induced toxicity in vivo?. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/A009