ICDSUPL1-A012

Volume: 1, 2022
1^st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT  – PLANT  – ANIMAL  – PRODUCT

Abstract number: A012

DOI: https://doi.org/10.24326/ICDSUPL1.A012

Published online: 26 April 2022

ICDSUPL, 1, A012 (2022)

The effect of humic substances on salinomycin residues in poultry

Simona Hriciková¹*, Ivona Kožárová¹, Slavomír Marcinčák¹

¹ Department of Food Hygiene, Technology and Safety, University of Veterinary Medicine and Pharmacy in Košice; Komenského 73, 041 81, Košice, Slovakia

^{* Corresponding author: simona.hricikova@student.uvlf.sk}

Abstract

As the worldwide use of antimicrobials increases, the presence of residues of pharmacologically active substances (RPAS) in animal products may endanger the health of consumers. The official controls are obligated by Regulation (EU) 2017/625 to ensure the safety of consumers, in particular on substances the use of which may lead to the presence of RPAS in food. In our work we focused on salinomycin (SAL), a coccidiostat classified under the Regulation (EC) No 1831/2003 as a feed additive used in animal nutrition. In order to reduce the consumption of antimicrobials, the use of natural alternatives in treatment and prevention is beneficial. Humic substances (HS) due to the presence of functional groups in their molecules may affect the presence of RPAS in animal products and also independently act as antimicrobials. An in vivo experiment was performed to evaluate this efficacy of the HS. 80 chickens were randomly divided into two groups of 40: Control (C) and Experimental (E). Chickens were fed with a commercial feed (CF): BR1 (1^st – 10^th day), BR2 (11^th – 30^th day, SAL) and BR3 (31^st – 37^th day). C group was fed a basal diet without the addition of any supplements. Diet of E group was supplemented with 0.7 % of HS from the first day of fattening. To detect SAL residues, two methods were used: 1. STAR – screening test for antibiotic residues using five bacterial strains, 2. Explorer 2.0 and Premi®Test, microbial inhibition tube tests (MITT) (in combination with an e-Reader). STAR was used to analyse chicken tissue samples (TS) (muscle, stomach, heart, liver, spleen and kidney). MITT were used for CF (BR1, BR2, and BR3) and muscle samples. In the case of using MITT in CF analysis, positive results were demonstrated for BR1 and BR2 CF samples. The BR3 CF sample came out negative. MITT showed negative results in case of muscle samples even in C or E groups. Analysis of SAL standards of 0.1, 0.5 and 1 µg/ml showed positive results only at the concentrations of 0.5 and 1 µg/ml. STAR method showed positive results in case of SAL standards at the concentrations of 0.5 and 1 µg/ml. In the case of TS, STAR showed the presence of SAL in all TS, with the highest values detected in the spleen and the lowest in the stomach. In all TS and both methods used, the values of detected SAL are significantly lower in the E group compared to the C group. These findings showed that HS reduce the SAL content in poultry TS and thus have a potential of decreasing RAPS in poultry products.

How to cite

S. Hriciková, I. Kožárová, S. Marcinčák, 2022. The effect of humic substances on salinomycin residues in poultry. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/A012