Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A021
DOI: https://doi.org/10.24326/ICDSUPL1.A021
Published online: 26 April 2022
ICDSUPL, 1, A021 (2022)
Immune response of rats fed a diet containing various sources of dietary fiber and copper nanoparticles
Aleksandra Marzec1, Radosław Smagieł1, Ewelina Cholewińska1, Anna Stępniowska1, Jerzy Juśkiewicz2, Katarzyna Ognik1
1 Department of Biochemistry and Toxicology, Faculty of Animal Science and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20–950 Lublin, Poland
2 Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland
* Corresponding author: aleksandra.marzec23@gmail.com
Abstract
The aim of this study is to verify the statement that the metabolic effect of copper nanoparticles (Cu-NPs) is strongly reliant on physiological function of different dietary fibre types. We hypothesized that a dietary combination of Cu-NPs with either a control inert (cellulose) or a prebiotic (inulin) or a viscous (pectin) or a bulking (psyllium) fibre would stimulate immune response of rats. Rats was fed for 6 weeks standard diet with dietary addition of two Cu-NP dosages (recommended and two times higher; 6.5 and 13 mg/kg diet) and combined with different types of dietary fibre; 6% of a diet (control – cellulose, inulin with prebiotic effect, psyllium with bulk effect, pectin with viscous effect; 10 groups, n=10 per group). Blood was collected from 10 rats from the group for the determination of immunological parameters: tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 2 (IL-2), acute phase protein (CRP), ceruloplasmin (Cp) and immunoglobulins IgA, IgM, IgY. Compared to the rats fed the control diet, partial replacement of cellulose by inulin or psyllium resulted in a statistically significant reduction in the levels of TNF-α, IgA, IgM, and IL-2 in the blood plasma of rats. In turn, the replacement of cellulose with pectin resulted in a decrease in the level of IgA, CRP and Cp in the blood plasma of rats. Increasing the dose of Cu-NPs from 6.5 to 13 mg/kg resulted in a decrease in the level of IgA, IgM and an increase in the level of IL-2 in the blood plasma of rats. No influence of the applied experimental factors on the level of pro-inflammatory interleukin IL-6 was found. The replacement of cellulose with 6% of inulin or psyllium favorably stimulates the immune system of the rats. Increasing the dose of copper nanoparticles increases the stimulation of the immune system, but determining whether this effect should be considered beneficial requires a wider range of research.
This work was supported by the National Science Centre, Grant No. 2021/41/B/NZ9/01104.
How to cite
A. Marzec, R. Smagieł, E. Cholewińska, A. Stępniowska, J. Juśkiewicz, K. Ognik, 2022. Immune response of rats fed a diet containing various sources of dietary fiber and copper nanoparticles. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/A021