Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A024
DOI: https://doi.org/10.24326/ICDSUPL1.A024
Published online: 26 April 2022
ICDSUPL, 1, A024 (2022)
Resistance to aminoglycosides in Escherichia coli strains isolated from animals
Marcelina Osińska1*, Aneta Nowakiewicz1, Sebastian Gnat1, Dominik Łagowski1, Aleksandra Trościańczyk1, Agata Hahaj-Siembida1
1 Department of Veterinary Microbiology, University of Life Sciences in Lublin, Akademicka 12, 20-033 Lublin, Poland
* Corresponding author: marcelina.osinska@up.lublin.pl
Abstract
Aminoglycosides are commonly used antibiotics in veterinary medicine. The result of the ECDC report showed an increase in the number of Escherichia coli strains resistant to aminoglycosides, fluoroquinolones and cephalosporins of the third generation in Poland. An interesting research group seems to be free-living animals, which, as not undergoing targeted therapy, should show the real level of resistance to antimicrobial substances present in the environment. The research material were 110 swabs from the rectum of foxes. Targeted isolation was used to isolate resistant E. coli strains using three types of MacConkey Agar supplemented with the following antibiotics: streptomycin, gentamicin and kanamycin. Molecular analysis of drug resistance of isolated E. coli strains was performed using the PCR method. The presence of the most common resistance genes among farm animals was checked: aac(3)-II, aac(3)-III (gentamicin), strA, strB (streptomycin) and aph(3′)-Ia, aph(3′)-IIa (kanamycin). Finally, 62 different E. coli isolates resistant to at least one aminoglycoside were selected for the study. The leading resistance to streptomycin was 64.5% (n = 40) and to kanamycin 22.6% (n = 14). Resistance to gentamicin was negligible and only two strains resistant to this antibiotic were found. Molecular analysis confirmed phenotypic resistance in most cases. Resistance to streptomycin was determined mainly by the presence of two strA and strB genes simultaneously (28/40 resistant), or a single strA gene (10/40 resistant). Kanamycin-resistant strains were characterized by the presence of the aph (3′)-Ia gene, and the tested resistance genes were not detected in three isolates. The aac(3)-II gene was found in both gentamicin-resistant strains. The obtained results indicate the need to monitor the level of resistance to commonly used antimicrobial substances in the group of free-living animals.
How to cite
M. Osińska, A. Nowakiewicz, S. Gnat, D. Łagowski, A. Trościańczyk, A. Hahaj-Siembida, 2022. Resistance to aminoglycosides in Escherichia coli strains isolated from animals. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/A024