ICDSUPL1-P004

Volume: 1, 2022
1^st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT  – PLANT  – ANIMAL  – PRODUCT

Abstract number: P004

DOI: https://doi.org/10.24326/ICDSUPL1.P004

Published online: 26 April 2022

ICDSUPL, 1, P004 (2022)

Alpha glucosidase inhibitory peptides derived from barley (Hordeum vulgare L.) proteins

Justyna Bucholska¹*, Anna Iwaniak¹, Małgorzata Darewicz¹, Marta Turło¹

¹ Department of Food Biochemistry, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Cieszyński Sq. 1, 10-726 Olsztyn, Poland

^{* Corresponding author:  justyna.bucholska@uwm.edu.pl}

Abstract

Cereals in ex. barley play the important role in human nutrition. Their consumption has a beneficial effect on human health e.g. reduce the risk of many chronic diseases (hypertension, cancer etc.). According to the literature, proteins can be a source of biologically active peptides in prevention of diet-related diseases. Peptides enzymatically released from food proteins, including those derived from barley, show variety of biological functions. The aim of the study was the determination of α-glucosidase inhibitory activity peptides derived from barley proteins. The analyses included an in silico analysis and in vitro protocols. The amino acid sequences of all proteins were acquired from the UniProt database. The bioinformatic analysis was carried out by using BIOPEP-UWM database. It included the calculation of profile of potential of α-glucosidase inhibitory activity of barley proteins, the frequency of α-glucosidase inhibitory fragments occurrence in protein sequence (A), simulation of proteolysis using human digestive enzymes to generate α-glucosidase inhibitors. Verification of in silico results included the extraction of barley proteins, enzymatic hydrolysis using the unified digestive protocol of Infogest and the determination of the α-glucosidase inhibitory activity of hydrolysates. The results showed that barley proteins may act as α-glucosidase inhibitors. This is indicated by the presence of fragments with α-glucosidase inhibitory peptides. Such peptides can also be released due to the action of digestive enzymes. Identification of α-glucosidase inhibitors in barley protein hydrolysates is yet to be determined.

This research was supported by a grant from National Science Centre in Poland (project no. DEC-2017/01/X/NZ9/00370). The participation of Dr. Justyna Bucholska in this conference was financially supported by the Minister of Education and Science under the program entitled “Regional Initiative of Excellence” for the years 2019-2022, Project No. 010/RID/2018/19, amount of funding 12.000.000 PLN.

How to cite

J. Bucholska, A. Iwaniak, M. Darewicz, M. Turło, 2022. Alpha glucosidase inhibitory peptides derived from barley (Hordeum vulgare L.) proteins. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/P004