Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: P007
DOI: https://doi.org/10.24326/ICDSUPL1.P007
Published online: 26 April 2022
ICDSUPL, 1, P007 (2022)
Insights into the genomes of Apilactobacillus kunkeeei strains isolated from flowers
Klaudia Gustaw1*, Anita Gorczyca1, Patrycja Wojdyło1
1Department of Biotechnology, Microbiology, and Human Nutrition, Faculty of Food Science and Biotechnology, University of Life Sciences in Lublin, Skromna 8, 20-704, Lublin, Poland
* Corresponding author: klaudiagustaw1@gmail.com
Abstract
Microorganisms typically have unique functional properties which are a result of a series of adaptations to the conditions of a given environment. The diversity of ecosystems has resulted in the evolutionary binding of the microorganism to its living environment. Lactic acid bacteria (LAB) are known to have adapted to a range of environments through gene acquisition or loss. In response not only to physical and chemical factors that promote the growth of these bacteria but also to unfavorable conditions, they have formed a unique repertoire of genes. Here, an environment rich in monosaccharides especially fructose can be specified, which has promoted the occurrence of fructophilic lactic acid bacteria (FLAB). Found in the nectars of flowers, fruits or even in the digestive tracts of insects, these bacteria are characterized by adaptation to fructose and high concentrations of sugars. The subjects of this study are three Apilactobacillus kunkeei strains belonging to the FLAB group. Two strains were isolated from flowers found in wastelands in Poland, then their genomes were sequenced using MinION and MiSeq. The genome sequences of the two newly isolated Apilactobacillus kunkeei strains, 7K11C and 7K4AA, are beyond the average length of the species, and furthermore, possessing plasmids, they increase the gene pool. A reference strain from the German DSMZ collection was also sequenced in the study, which, due to its WGS status, updated this record at NCBI. Analysis of these genomes provided insights into their fructophilicity, characteristics as well as unique features, supporting a distinctive model for the evolution of fructophilic lactic acid bacteria genomes. The whole-genome shotgun projects have been deposited in NCBI GenBank, and can be found by Biosample numbers SAMN20804187 (A. kunkeei strain 7K11C), SAMN20557569 (A. kunkeei strain 7K4AA), SAMN20557570 (A. kunkeei strain DSMZ 12361).
This work was financially supported by the National Science Centre, Preludium 15 Program (grant 2018/29/N/NZ9/00985), Poland.
How to cite
K. Gustaw, A. Gorczyca, P. Wojdyło, 2022. Insights into the genomes of Apilactobacillus kunkeeei strains isolated from flowers. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/P007