Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: P010
DOI: https://doi.org/10.24326/ICDSUPL1.P010
Published online: 26 April 2022
ICDSUPL, 1, P010 (2022)
Immunomodulatory activity of glucooligosaccharides obtained from fungal cell wall α-1,3-glucans
Nataliia Kosheva1*, Sylwia Stączek1, Agnieszka Zdybicka-Barabas1, Małgorzata Cytryńska1, Adrian Wiater1 and Monika Koziej1
1Department of Immunobiology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-030 Lublin, Poland
* Corresponding author: nataliia.kosheva21@gmail.com
Abstract
All living organisms, including humans, are at risk of attack by pathogenic microorganisms. Therefore, to better cope with infections, many substances with immunomodulatory activity are used. An excellent source of these is the fungal cell wall. Its ingredients include polysaccharides, mainly β-1,3-glucans, which activate the mechanisms of innate immunity in animals. From polysaccharides, by chemical or enzymatic hydrolysis, oligosaccharides containing up to 20 glucose subunits or other simple sugars can be obtained. Many of them have immunostimulatory, anti-cancer, anti-inflammatory effects and are also considered as prebiotics. An important component of the cell wall of some fungi is α-1,3-glucan. A rich source of α-1,3-glucan are the bracket fungi: Fomitopsis betulina and Laetiporus sulphureus. Immunomodulatory effect of GOS obtained from α-1,3-glucan isolated from the cell wall of A. niger, F. betulina and L. sulphureus on a model organism, Galleria mellonella caterpillars was investigated. An increase in PO activity was found in the hemolymph of GOS-injected insects, especially injected with GOS from F. betulina and L. sulphureus. The lysozyme activity in the hemolymph reached the highest value 48 h post-treatment with L. sulphureus GOS. The administration of GOS from F. betulina and L. sulphureus resulted in an increase in anti-E. coli activity in G. mellonella hemolymph, especially during 6-24 h. The antifungal activity appeared only during 24-72 h and 24-48 h post-injection with GOS from F. betulina and L. sulphureus, respectively. Moreover, electrophoretic analysis showed that bands below 6 kDa, indicating the presence of antimicrobial peptides, appeared in the hemolymph of these larvae. The obtained results indicate that GOSs trigger an immune response in insects. Moreover, depending on their origin, they show different immunomodulatory properties.
How to cite
N. Kosheva, S. Stączek, A. Zdybicka-Barabas, M. Cytryńska, A. Wiater, M. Koziej, 2022. Immunomodulatory activity of glucooligosaccharides obtained from fungal cell wall α-1,3-glucans. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/P010