Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: P011
DOI: https://doi.org/10.24326/ICDSUPL1.P011
Published online: 26 April 2022
ICDSUPL, 1, P011 (2022)
Comparative analysis of three selected transcripts of sugar beet (Beta vulgaris L. subsp. vulgaris) after 7 days of drought and maintained under optimal irrigation
Karolina Różaniecka1*, Michał Nowak1 and Krzysztof Kowalczyk1
1Institute of Plant Genetics, Breeding and Biotechnology, University of Life Sciences in Lublin, Akademicka 15, 20-950 Lublin, Poland
* Corresponding author: karolina.rozaniecka@up.lublin.pl
Abstract
Water scarcity is currently one of the most important factors limiting crop yields and food production worldwide. Over the last few years, a steady trend including increasing temperatures and decreasing rainfall, which adversely affects plant growth and development has been observed. The only solution to reduce the adverse effects of water scarcity on cultivated plants is to grow varieties with increased tolerance to this type of stress. Drought tolerance is a multigene and multifactorial trait expressed by morphological, anatomical and metabolic changes. Sugar beet is one of the most important root crops cultivated in Poland, being the main raw material for sugar production. Cultivation of sugar beet on an industrial scale began in the early 19th century. Poland is currently one of the most important sugar beet producers in the world with a production of over 14.3 million tons. In the presented study six sugar beet (Beta vulgaris L. subsp. vulgaris) genotypes were analyzed. The seeds of the tested forms were sown directly into the substrate and maintained under optimal conditions in a phytotron until the 2-week-old seedling stage. Then the plants were divided into two sets: half of the plants were kept under optimum substrate moisture conditions and half were kept under reduced substrate moisture conditions for 7 days. After the 7-day drought, the plant material was collected and immediately frozen in liquid nitrogen and placed at -80°C. The collected plant material was subjected to isolation of total RNA by manual method using TRIzol reagent. The isolated RNA samples were measured using a NanoDrop spectrophotometer to check purity and separated on a 2% agarose gel to check integrity. Subsequently, obtained RNA was reverse transcribed to cDNA. The cDNA samples were used as a template for qPCR reactions. The level of expression for three genes encoding actin, tubulin and translation initiation factor was determined using SYBR Green based reagent kit.
How to cite
K. Różaniecka, M. Nowak, K. Kowalczyk, 2022. Comparative analysis of three selected transcripts of sugar beet (Beta vulgaris L. subsp. vulgaris) after 7 days of drought and maintained under optimal irrigation. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/P011