Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: T045
DOI: https://doi.org/10.24326/ICDSUPL1.T045
Published online: 26 April 2022
ICDSUPL, 1, T045 (2022)
Assessment of biological activity of Epilobium angustifolium L. (Fireweed)
Klaudia Kowalik1*, Magdalena Polak-Berecka1, Monika Prendecka-Wróbel2, Dominika Pigoń-Zając2, Iwona Niedźwiedź1, Dominik Szwajgier1, Ewa Baranowska-Wójcik1, and Adam Waśko1
1 Department of Biotechnology, Microbiology and Human Nutrition, University of Life Sciences in Lublin, Skromna 8, 20-704 Lublin, Poland
2 Chair and Department of Human Physiology, Medical University of Lublin, Radziwiłłowska 11, 20-080 Lublin, Poland
* Corresponding author: klaudia.kowalik@up.lublin.pl
Abstract
Epilobium angustifolium L. is a plant belonging to the Onagraceae family. It grows in Europe, America and Asia. The biological activity of Epilobium angustifolium L. was assessed after the in vitro digestion process. The studies were carried out using the human colon epithelial cell line CCD 841 CoTr and human colorectal adenocarcinoma HT-29 and CaCo-2 cell lines. The determination of the phenolic compounds in the aqueous extract after digestion was carried out with HPLC-ESI-QTOF-MS/MS. The following methods were used to evaluate the cytotoxic and antiproliferative properties and to check the effect of the extract on the number and appearance of cells: Neutral Red uptake assay, MTT assay, label-free Electric Cell-Substrate Impedance Sensing cytotoxicity assay and May-Grünwald-Giemsa staining. The effect of fireweed extract on bacterial growth was also investigated. The experiment was carried out with gram-negative strains and gram-positive bacteria. After analyzing the results, it was found that the extract acted mainly on growth intestinal cancer cells HT-29. Growth inhibition may be related to the effects of quercetin and kaempferol, which was the most abundant phenolic compound in the extract after in vitro digestion. The biggest decrease in proliferation (almost 80%) compared to control was observed in the treated HT-29 line extract at a concentration of 250 μg/ml. Fireweed extract did not affect the growth of most bacteria. It only significantly inhibited the growth of E. coli. The biological activity of the extract after in vitro digestion shows that this process does not affect the cytotoxic and antiproliferative properties. It can be concluded that in the future, after more detailed research, fireweed extract could be used in the prevention of colon cancer.
How to cite
K. Kowalik, M. Polak-Berecka, M. Prendecka-Wróbel, D. Pigoń-Zając, I. Niedźwiedź, D. Szwajgier, E. Baranowska-Wójcik, A. Waśko, 2022. Assessment of biological activity of Epilobium angustifolium L. (Fireweed). In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/T045