Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A011
DOI: https://doi.org/10.24326/ICDSUPL2.A011
Published online: 19 April 2023
ICDSUPL, 2, A011 (2023)
Molecular differences in the mitochondrial DNA in dogs with multiple malignant tumours
Krzysztof Kowal1*, Angelika Tkaczyk-Wlizło1, Kaja Ziółkowska1, Klaudia Lasota1, Paweł Grychnik1, Brygida Ślaska1
1 Institute of Biological Basis of Animal Production, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences, Akademicka 13, 20-950 Lublin, Poland
* Corresponding author: krzysztof.kowal@up.lublin.pl
Abstract
The aim of this study was to identify molecular defects caused by mutations in mitochondrial DNA in multiple canine tumours. We analysed the whole mitochondrial genome (mtDNA) of a dog with two mammary carcinoma complexus G1 tumours of a different size (5×8 cm and Ø 1 cm) as well as the mtDNA obtained from of a dog with sertolioma and epithelioma glandulae perianales tumours. In the case of the dog with two carcinoma complexus G1 tumours localised on mammary glands differing in the tumour size, 36 molecular alterations were found. Among these 36 changes, four mutations (m.16178A>G, m.16208A>G, m.16248A>G, m.16298A/G) were found in the VNTR region of the sequence obtained from the bigger tumour, and four mutations (m.16158A/G, m.16168A/G, m.16188G/A, m.16218G/A) were found in the VNTR region of the sequence obtained from the smaller tumour. There were no mutations in the coding regions of the mitochondrial genome. Three polymorphisms: m.7308A>G (COX2), m.10204C>T (ND4L), and m.16507T>A (control region) were identified, from which one of them led to a nonsynonymous alteration in the COX2 protein (p.Asn92Ser). There were 120 molecular alterations, from which 100 were polymorphisms found in the dog with two different tumours of the same epithelial origin in two different tissues. Four of the twelve mutations identified in the samples from this dog were found in sertolioma tumour, six in epithelioma glandulae perianales, and two mutations in the blood sample. The heteroplasmic mutation in the ND6 gene led to a heteroplasmy in the amino acid sequence of the ND6 protein (p.Pro26=/Leu). The number of mutations in tumours differing in size was the same; however, the smaller tumour had more heteroplasmic mutations that may be linked with higher genomic instability. The alterations in the VNTR region may play an important role in carcinogenesis leading to disturbances in the replication or transcription of mtDNA genes.
This research was funded by the National Science Centre in Poland, grant number 2019/35/B/NZ5/00775 and financed by the University of Life Sciences in Lublin, grant number ZIB/MN-3/ZiR/21.
How to cite
K. Kowal, A. Tkaczyk-Wlizło, K. Ziółkowska, K. Lasota, P. Grychnik, B. Ślaska, 2023. Molecular differences in the mitochondrial DNA in dogs with multiple malignant tumours. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A011