Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A016
DOI: https://doi.org/10.24326/ICDSUPL2.A016
Published online: 19 April 2023
ICDSUPL, 2, A016 (2023)
The effect of copper nanoparticles and a different source of dietary fiber in the diet on the expression of genes involved in maintaining the integrity of the small intestine in the rat
Aleksandra Marzec1*, Ewelina Cholewińska1, Przemysław Sołek1, Krzysztof Tutaj1, Jerzy Juśkiewicz2, Katarzyna Ognik1, Ramutė Mišeikienė3
1 Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences, 20-950 Lublin, Poland
2 Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland
3 Institute of Animal Rearing Technologies, Faculty of Animal Science, Lithuanian University of Health Sciences, Lithuania
* Corresponding author: aleksandra.marzec@up.lublin.pl
Abstract
The aim of the study was to verify the hypothesis regarding the effect of recommended Cu/kg level (6.5 mg) or two times higher (13.0 mg) level of CuNPs in the diet in combination with different types of dietary fiber – cellulose (control), inulin, pectin or psyllium may effect on the expression of genes involved in maintaining the integrity of the small intestine. One hundred, healthy male Wistar rats 6-week aged were divided to 10 groups. The first two groups were fed a control diets contained α-cellulose as a control dietary fiber and a mineral mixture with standard or enhanced content of Cu from the standard source CuCO3 (6.5 or 13 mg/kg diet). Other groups were fed diet supplemented with two CuNPs doses (6.5 or 13 mg/kg diet) and combined with four different types of dietary fibre – α-cellulose (control dietary fibre), pectin, inulin or psyllium. The control dietary fiber, α-cellulose, was added at 8% of a diet while the experimental fiber preparations (pectin, inulin and psyllium) were added at 6% of a diet at the expense of cellulose preparation. After feeding period, rats were sacrificed and small intestine samples were collected and the expression of selected genes responsible for intestinal integrity such as occludin (OCLN), 8-oxoguanine glycosylase (OGG1), trefoil factor 2 (TFF2) and zonula occludens-1 (ZO-1) was measured by real-time PCR technique. The study showed that replacing the recommended level of CuCO3 with an equivalent amount of CuNPs in the diet of rats resulted in a decrease in the level of TFF2 and ZO-1, without changes in the level of OCLN expression. However, there was no effect of a two-fold increase in the level of CuNPs in the diet in relation to the dietary recommendations on the level of expression of the analyzed genes of the intestinal barrier in rats. In the small intestine of rats receiving a combination of CuNPs and psyllium in the diet, an increase in the expression level of ZO-1 was observed compared to the group receiving cellulose as a standard source of dietary fiber. The obtained results confirmed the assumption that the combination of CuNPs with sources of dietary fiber other than cellulose may affect the expression of genes responsible for maintaining intestinal integrity. The obtained results suggest that the dietary combination of CuNPs and psyllium has the most beneficial effect on the expression of intestinal integrity genes.
This research was supported by the National Science Centre, Poland, Grant No. 2021/41/B/NZ9/01104.
How to cite
A. Marzec, E. Cholewińska, P. Sołek, K. Tutaj, J. Juśkiewicz, K. Ognik, R. Mišeikienė, 2023.The effect of copper nanoparticles and a different source of dietary fiber in the diet on the expression of genes involved in maintaining the integrity of the small intestine in the rat. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A016