Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A017
DOI: https://doi.org/10.24326/ICDSUPL2.A017
Published online: 19 April 2023
ICDSUPL, 2, A017 (2023)
The effect of copper nanoparticles and a different source of dietary fiber in the diet on selected indicators of small intestine integrity and histological image of the small intestine in the rat
Aleksandra Marzec1*, Ewelina Cholewińska1, Bartosz Fotschki2, Jerzy Juśkiewicz2, Katarzyna Ognik1
1 Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences, 20-950 Lublin, Poland
2 Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-748 Olsztyn, Poland
* Corresponding author: aleksandra.marzec@up.lublin.pl
Abstract
The aim of the study was to verify the hypothesis regarding the effect of recommended Cu/kg level (6.5 mg) or two times higher (13.0 mg) level of CuNPs in the diet in combination with different types of dietary fiber – cellulose (control), inulin, pectin or psyllium may effect on selected indicators of small intestine integrity and histological image of the small intestine in the rat. One hundred, healthy male Wistar rats 6-week aged were divided to 10 groups. The first two groups were fed a control diets contained α-cellulose as a control dietary fibre and a mineral mixture with standard or enhanced content of Cu from the standard source CuCO3 (6.5 or 13 mg/kg diet). Other groups were fed diet supplemented with two CuNPs doses (recommended and two times higher; 6.5 and 13 mg/kg diet, respectively) and combined with four different types of dietary fibre – α-cellulose (control dietary fibre), pectin, inulin or psyllium. The control dietary fibre, α-cellulose, was added at 8% of a diet while the experimental fibre preparations (pectin, inulin and psyllium) were added at 6% of a diet at the expense of cellulose preparation. After feeding period, rats were sacrificed and samples of blood and small intestine were collected. In the blood plasma the level of lactic acid (LA) and diamine oxidase (DAO) was determined. The pH values, dry matter and viscosity were measured in the ileal digesta. In the tissue of small intestine the level of 8-hydroxydeoxyguanosine (8-OHdG), apurinic/apyrimidinic endonuclease 1 (APE-1), 8-oxoguanine DNA glycosylase (OGG1), lactic acid (LA), diamine oxidase (DAO), caspase 3 and caspase 8 were determined. Additionally, small intestine was subjected to histopathological examination. Replacing CuCO3 with CuNPs in the diet of rats resulted in a decrease in the LA content in the blood plasma and small intestine, as well as a decrease in the level of caspases 3 and 8 and an increase in the level of 8-OHdG in the intestinal tissue. Increasing the level of CuNPs from 6.5 to 13 mg/kg of the diet only resulted in an increase in the level of DAO in the blood of rats. The addition of pectin or psyllium to the diet resulted in an increase in the ileal viscosity, while the addition of pectin and inulin increased the acidity of the illeum content. All tested forms of dietary fiber contributed to the reduction of dry matter of illeum. The addition of pectin and inulin to the diet of rats resulted in a decrease in the level of DAO both in the blood plasma and in the small intestine. The addition of pectin to the diet of rats resulted in a decrease in the level of caspase 3 in the tissue of the small intestine. The histopathological examination did not show any negative changes in the morphology of the small intestine of rats receiving a combination of the recommended and higher level of CuNPs and all tested source of dietary fiber. The obtained results suggest that dietary fiber supplementation in the diet of rats effectively protects the small intestine against the potentially harmful, oxidative effects of CuNPs by strengthening the intestinal barrier, which ultimately may translate into the regulation of their beneficial metabolic effect.
This research was supported by the National Science Centre, Poland, Grant No. 2021/41/B/NZ9/01104.
How to cite
A. Marzec, E. Cholewińska, B. Fotschki, J. Juśkiewicz, K. Ognik, 2023. The effect of copper nanoparticles and a different source of dietary fiber in the diet on selected indicators of small intestine integrity and histological image of the small intestine in the rat. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A017