Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A026
DOI: https://doi.org/10.24326/ICDSUPL2.A026
Published online: 19 April 2023
ICDSUPL, 2, A026 (2023)
Effect of feeding diet containing coccidiostat and administration of antibiotics to turkeys in the first days after hatching on blood levels of antibiotics
Radosław Smagieł1*, Paulina Osowska1, Krzysztof Tutaj1, Jan Jankowski2, Katarzyna Ognik1
1 Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
2 Department of Poultry Science and Apiculture, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland
* Corresponding author: radoslaw.smagiel@up.lublin.pl
Abstract
The aim of the study was to determine whether antibiotics administered during the early rearing period of turkeys undergo biotransformation, as a result of which their level will be undetectable at 8 weeks of age. The experiment was conducted on 1152 one-day-old turkeys divided into 6 treatments: CM(-), EM(-), DM(-), CM(+), EM(+), DM(+). Turkeys in the M(-) groups did not receive monensin in the diet, while those in the M(+) groups received monensin at a dose of 90 mg/kg of feed. Turkeys in groups C, received no antibiotic supplement (control groups), while those in groups E received enrofloxacin at a dose of 10 mg/kg BW for the first 5 days of life, and those in groups D received doxycycline at a dose of 50 mg/kg BW for the first 5 days of life. The birds were vaccinated against APV and NDV on day 1 of life and against ORT on day 28 of life. On days 7 and 56, blood was collected from 8 birds in the group to evaluate monensin, enrofloxacin, and doxycycline levels. Analyzes were performed using an ion mobility spectrometer with a high-pressure liquid chromatograph (UPLC/QQQ-IMS-IT) in triplicate. The presence of antibiotics in the blood collected from turkeys from the control group was not found. In the blood of 7-day-old turkeys receiving enrofloxacin, the presence of this antibiotic was found at a level of about 120 μg/ml, and the level of this antibiotic decreased by about 87% by 56 days of age. In the blood of 7-day-old turkeys, which were fed a diet supplemented with monensin and at the same time received enrofloxacin in the first days of life, the presence of both antibiotics was found (about 67 μg/ml enrofloxacin and 5.7 μg/ml monensin), and in the blood of 56-day-old turkeys of enrofloxacin decreased by about 94%, and the concentration of monensin increased by 220%. Interestingly, in the case of self-administered monensin, its blood levels in 7 and 56-day-old turkeys turned out to be quite similar. In the blood of 7-day-old turkeys receiving doxycycline in the first days of life, its level was about 1230 μg/ml, and on the 56th day it decreased by about 99%. Nutrition with a diet containing monensin and early administration of doxycycline caused a similar effect as in the case of doxycycline alone (a decrease of about 99% at 56 days of age). In conclusion, it should be stated that the concentration of antibiotics administered in the first five days of life to turkeys is reduced in blood by about 80-99% in 8-week-old turkeys. Enrofloxacin and doxycycline administered in the early rearing period is not fully biotransformed until the 8th week of life of the turkeys, however, it should be noted that doxycycline is biotransformed to a greater extent than enrofloxacin.
This work was supported by the National Science Centre in Poland, Grant No. 2020/39/B/NZ9/00765.
Investments in Scientific Activity were co-financed from the State Budget. Ion Mobility Spectrometer with High Pressure Liquid Chromatograph (UPLC/QQQ-IMS-IT).
How to cite
R. Smagieł, P. Osowska, K. Tutaj, J.Jankowski, K. Ognik, 2023. Effect of feeding diet containing coccidiostat and administration of antibiotics to turkeys in the first days after hatching on blood levels of antibiotics. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A026