Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A031
DOI: https://doi.org/10.24326/ICDSUPL2.A031
Published online: 19 April 2023
ICDSUPL, 2, A031 (2023)
Effect of dietary protein on liver homeostasis of Eurasian perch
Wiktoria Wiechetek1*, Dobrochna Adamek-Urbańska1
1 Department of Ichthyology and Biotechnology in Aquaculture, Institute of Animal Science; Warsaw University Of Life Sciences, Ciszewskiego 8 St., bldg. 23, 02-786 Warsaw, Poland
* Corresponding author: wiktoria_wiechetek@sggw.edu.pl
Abstract
Due to growing market demand and success in intensive fish production, Eurasian perch (Perca fluviatilis) is now considered a candidate species for diversification of European aquaculture. It is therefore necessary to develop efficient diets that would allow the commercial production of nutritious fish for consumers. The aim of the performed feeding experiment was to determine the effect of dietary protein level on histology and enzymatic activity of the liver of juvenile Perca fluviatilis. A 10 week trial was conducted using 38%, 42%, 46% and 50% protein feed. For histological analysis, liver samples were taken from 12 individuals from each feeding group and processed using a standard paraffin-embedding procedure. The microtome-cut sections were stained with either hematoxylin and eosin (HE) or AB/PAS (Alcian Blue – Schiff’s Reagent with Periodic Acid), and were subjected to microscopic analyses. In order to determine liver homeostasis, frozen tissue samples were homogenized and the spectrophotometric analysis of the following enzymes was performed: alkaline phosphatase, acid phosphatase and superoxide dismutase (SOD). The histological structure of the liver was characterized by irregular steatosis. Only the group fed the 50% protein diet exhibited highly abundant glycogen and little fatty vacuolization of hepatocytes compared to the other groups. Increased phosphatase activity was also observed in fish fed diets with protein content lower than 50%. The 46% protein diet group had also increased hepatic SOD activity. Based on the carried out analyses, it was concluded that the dietary protein level for rearing juvenile P. fluviatilis in RAS should not be lower than 50%.
How to cite
W. Wiechetek, D. Adamek-Urbańska, 2023. Effect of dietary protein on liver homeostasis of Eurasian perch. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A031