Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A038
DOI: https://doi.org/10.24326/ICDSUPL2.A038
Published online: 19 April 2023
ICDSUPL, 2, A038 (2023)
Impact of anaesthetics on the canine choroid assessed with SD-OCT
Jowita Zwolska1*, Ireneusz Balicki1, Agnieszka Noszczyk-Nowak2, Mateusz Szadkowski1, Łukasz Mazurek3, Dagmara Gadomska1
1 Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
2 Department of Internal Medicine and Clinic of Diseases of Horses, Dogs and Cats, Wroclaw University of Environmental and Life Sciences, pl. Grunwaldzki 47, 50-366 Wroclaw, Poland
3 Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences in Lublin, Gleboka 30, 20-612 Lublin, Poland
* Corresponding author: jowita.zwolska@gmail.com
Abstract
The choroid is an anatomical structure with the primary function of supplying oxygen and nutrients to the retina. It is primarily composed of blood vessels, nerves, and pigment cells. Spectral-domain optical coherence tomography (SD-OCT) is a non-invasive, non-contact method that provides real-time in vivo imagery of the choroid. The assessment of the choroid examined in clinically healthy animals undergoing anaesthesia is required for further studies regarding vascular alterations. To determine the impact of anaesthetics on the canine choroid as a consequence of their effect on the cardiovascular system. The study was performed on 10 beagles aged 3-5 years, weighing 13-15 kg. The dogs were anesthetized with medetomidine (0,04 mg/kg b.w.) followed by ketamine (5 mg/kg b.w.) in intramuscular injections. Before administration of the anaesthetic agents (Control group), 15 minutes after medetomidine (Med group) and 15 minutes (Ket group), and an hour after ketamine injections (Post group), all dogs underwent ophthalmic examination including SD-OCT and cardiovascular examination. Measurements of choroidal layers: RPE-Bruch membrane-choriocapillaris complex (RPE-BmCc) with tapetum lucidum in the tapetal fundus, medium-sized vessel layer, (MSVL), large vessel layer with lamina suprachoroidea (LVLS) and whole choroidal thickness (WCT) were performed dorsally(D), ventrally(V) in the distance 5000-6000 μm, temporally(T) and nasally(N), in the distance 6500-7000 μm to the optic disc on enhanced depth scans. The measurements were conducted temporally and nasally both in tapetal(Tt,Nt), and nontapetal(Tnt,Nnt) fundus. Echocardiography was performed and mean arterial pressure, blood flow through the aorta and pulmonary artery were measured. Statistical analyses were performed using analysis of variance (ANOVA). WCT was statistically significantly greater in all nontapetal regions respectively in V, Tnt, and Nnt (mean [μm]) – in Ket (101.75; 117.00; 110.80) than in Control (87.55; 104.95; 101.10). In the V region, LVLS and WCT were respectively statistically significantly greater in Med (69.00; 95.35), Ket (72.05; 101.75), and Post (68.85; 95.90) than in Control (61.40; 87.55). Medetomidine and ketamine affect the thickness of choroidal layers and WCT.
How to cite
J. Zwolska, I. Balicki, A. Noszczyk-Nowak, M. Szadkowski, Ł. Mazurek, D. Gadomska, 2023. Impact of anaesthetics on the canine choroid assessed with SD-OCT. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A038