Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: B009
DOI: https://doi.org/10.24326/ICDSUPL2B009
Published online: 19 April 2023
ICDSUPL, 2, B009 (2023)
When Mycobacterium is older than you. Can it be recovered?
Dominik Łagowski1*, Marta Dec2, Klaudia Świca3
1 Freelancer, Lublin, Poland
2 Department of Veterinary Prevention and Avian Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-033 Lublin, Poland
3 Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin,20-033 Lublin, Poland
* Corresponding author: dlagowsky@gmail.com
Abstract
Long-term storage of bacterial strains that they retain their viability is difficult in many cases. In the scientific literature, there are more and more descriptions of methods for the recovery of bacterial strains, in particular reference strains, which are usually very expensive and poorly maintained on the media. The aim of this study was to check the proprietary microbiological method aimed at recovering various species of Mycobacterium from dried Lowenstein-Jansen (LJ) medium that are more than 20 years old. The following strains were used in the study: M. marinum, M. intracellulare, M. phlei, M. scrofulaceum and the reference strain Mycobacterium H37Ra (BSL2). A wild strain of M. vaccae was used as a control strain. There were 3 dried slants of each strain, the age of which was estimated at 25 years based on the information on the tubes. Author’s BFO liquid medium was used as the recovery medium. Dried LJs were flooded with liquid BFO medium and incubated at 370C for 30 days. Then 1 ml of medium was taken and resuspended in sterile BFO medium and incubated again for 30 days at 370C. After this time, the presence of sediment at the bottom of the tubes, the presence of turbidity was assessed. Microbiological purity was checked on Columbia medium with 5% sheep blood (72h/370C). The stability of the recovered strains was checked on Stonebrink medium with PACT (21days/370C), where the characteristic growth of Mycobacteria was assessed. Overall, all tested strains were recovered. In the case of the M. intracellulare strain, recovery was successful in one case. For the tested strains, the percentage of recovery was 73.3%. The use of the BFO medium for the recovery of Mycobacterium strains seems to be a good alternative to the standard media used for the cultivation of Mycobacteria. The use of BFO requires further validation due to the small number of strains that have been stored for more than 20 years on slants.
How to cite
D. Łagowski, M. Dec, K. Świca, 2023. When Mycobacterium is older than you. Can it be recovered?. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2B009