Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: B011
DOI: https://doi.org/10.24326/ICDSUPL2B011
Published online: 19 April 2023
ICDSUPL, 2, B011 (2023)
Comparison of the significance of dietary copper nanoparticles and copper carbonate on limiting neurodegenerative changes in the brain and gut of rats
Monika Cendrowska-Pinkosz1, Magdalena Krauze2, Jerzy Juśkiewicz3, Michalina Pinkosz1*, Katarzyna Ognik2
1 Department of Human Anatomy, Medical University of Lublin, Jaczewski 4, 20-090 Lublin, Poland
2 Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
3 Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748, Olsztyn, Poland
* Corresponding author: pinkoszmichalina00@gmail.com
Abstract
It was established that the neuroprotective properties of dietary administered copper can be improved when the traditional carbonate (CuCO3) form is replaced with copper nanoparticles (CuNPs). The neuroprotective effect depends on both the administered dose and form of copper. The aim of this study was to assess the interaction between two different forms and doses of copper present in the rat diet on the levels of chosen biochemical markers depicting neurodegenerative changes in the brain and the gut. The experiment was performed using healthy outbred male Wistar rats fed standard semi-purified rat diet with two doses of Cu (standard and two times higher), used as CuCO3 and dietary addition of two CuNPs dosages (recommended and two times higher). The rats aged 6-wk were randomly divided into 4 groups with n = 10 per one group. All animal care and experimental protocols were complied with the current laws governing animal experimentation in the Republic of Poland and by an ethical committee according to the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes, Directive 2010/63/EU for animal experiments, and will be approved by the Local Ethics Committee for Animal Experiments. To measure the presence and stage of advancement of neurodegenerative processes in the nervous tissues in the brain and the gut we examined the levels of: AChE – acetylcholinesterase; βAP – β amyloid protein; GAChE – glycosylated acetylcholinesterase; LRP1 – low density lipoprotein receptor-related protein 1; Tau – Tau protein; p-Tau – hyperphosphorylated Tau protein; CAMK2a – Calcium/Calmodulin Dependent Protein Kinase II Alpha; ABC – ATP Binding Cassette Transporter and the level of DNA methylation. The results of two-factor statistical analysis showed that the source of copper did not influence the fact that a higher dosage of copper in the form of CuNP increased the amount of AChE, while copper administered in the form of CuCO3 decreased the levels of AChE. The application of two different doses of copper (6,5 mg and 13 mg) significantly varied the level of neurometabolic markers in the brain and the gut of the rat. The highest level of AChE was observed in tissues of rats that were given 6,5mg of copper in their diet. Considerably lower levels of GAChE and βAP were annotated both in the brain and the gut of rats that were given 6,5mg of copper in their diet regardless of the chemical form. A smaller dosage of copper (6,5 mg) regardless of its chemical form caused a decrease in the concentration of p-Tau and CAMK2a in the rat brain and simultaneous increase in the concentration of ABC and LRP1 in the rat brain. The level of p-Tau was smaller in the gut tissue of rats who were administered 6,5 mg of copper in comparison with the animals who received 13 mg of copper in their diet. The study showed that the application of CuCO3 and CuNPs influenced the variability of neurometabolic markers in the rat brain and gut. The application of CuNPs had a greater influence on decreasing the levels of GAChE and βAP both in the rat brain and gut. The administration of CuNPs regardless of the dosage decreased the levels of p-Tau in the rat brain. The administration of CuNPs could increase the levels of AChE and LRP1 in the rat gut. The results of our study indicate the possibility of a better utilization of the neuroprotective properties of dietary administered copper if the carbonate form is exchanged for CuNPs. The dosage of 6,5 mg has a more beneficial impact on decelerating the occurrence of neurodegenerative changes. The evidence of neuroprotective properties of CuNPs in the dosage of 6,5 mg is shown in the increased levels of AChE in the brain, decreased levels of toxic GAChE and βAP in the brain as well as decreased levels of p-Tau and increased levels of LRP1 in the gut.
The study was financed in the project of NCN: 2021/41/B/NZ9/01104.
How to cite
M. Cendrowska-Pinkosz, M. Krauze, J. Juśkiewicz, M. Pinkosz, K. Ognik, 2023. Comparison of the significance of dietary copper nanoparticles and copper carbonate on limiting neurodegenerative changes in the brain and gut of rats. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2B011