Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A018
DOI: https://doi.org/10.24326/ICDSUPL3.A018
Published online: 24 April 2024
ICDSUPL, 3, A018 (2024)
Is the effect of copper nanoparticles on the blood morphology of rats dependent on the diverse physiological functions of dietary fiber?
Aleksandra Marzec1*, Bartosz Fotschki2, Jerzy Juśkiewicz2, Bartosz Kwiatkowski3, Piotr Kiszowara3, Katarzyna Ognik1
1 Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
2 Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10–748 Olsztyn, Poland
3 Department of Cardiology, Dr. Tytus Chałubiński Specialist Hospital in Radom, Lekarska 4, 26-610 Radom, Poland
* Corresponding author: aleksandra.marzec@up.lublin.pl
Abstract
The aim of the study was to determine whether the effect of copper nanoparticles (Cu-NP) on morphological parameters depends on the physiological functions of various types of dietary fiber. We hypothesized that a dietary combination of Cu-NPs with either a control inert (cellulose) or a prebiotic (inulin) or a viscous (pectin) or a bulking (psyllium) fiber would affect white and red blood cell indices. Rats was fed for 6 weeks standard diet with dietary addition of two Cu-NP dosages and combined with different types of dietary fibre. Groups C and CH were fed a control diet with standard and enhanced Cu content in the mineral mixture (6.5 and 13 mg/kg from CuCO3, respectively) with 8% of cellulose as dietary fibre source; groups CN and CNH were fed diets with supplementation of Cu-NP (6.5 and 13 mg/kg from Cu-nanoparticles, respectively) with 8% of cellulose dietary fibre source; groups PN and PNH were fed diets with supplementation of Cu- NP (6.5 and 13 mg/kg from Cu-nanoparticles, respectively) with 2% of cellulose and 6% of pectin dietary fibre source; groups JN and JNH were fed diets with supplementation of Cu-NP (6.5 and 13 mg/kg from Cu-nanoparticles, respectively) with 2% of cellulose and 6% of inulin dietary fibre source; groups SN and SNH were fed diets with supplementation of Cu-NP (6.5 and 13 mg/kg from Cu-nanoparticles, respectively) with 2% of cellulose and 6% of psyllium dietary fibre source. Blood was collected from 10 rats from the group for the determination of hematological parameters. Higher levels of WBC and lymphocytes were found in the blood of rats from the SN group. Compared to the CH control group, in the SNH group there was a decrease in mean corpuscular hemoglobin (MCH), while an increase in the percentage of red cell distribution width (RDWc) and mean platelet volume (MPV). Two-way analysis of variance showed that, compared to the use of cellulose (standard fiber), the use of pectin or inulin resulted in a reduction in the levels of WBC and LYM in the blood of rats (P = 0.015 and P=0.011 respectively). There was no influence of main effects, i.e. the type of dietary fiber used and the dose of CuNPs on the level of red blood cell indicators and platelets. The obtained results allow us to assume that the supply of fiber in the diet may beneficially reduce pro-inflammatory processes.
This work was supported by the National Science Centre in Poland, Grant No. 2021/41/B/NZ9/01104.
Keywords: copper, nanoparticles, dietary fiber
How to cite
A. Marzec, B. Fotschki, J. Juśkiewicz, B. Kwiatkowski, P. Kiszowara, K. Ognik, 2024. Is the effect of copper nanoparticles on the blood morphology of rats dependent on the diverse physiological functions of dietary fiber?. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.A018