Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A031
DOI: https://doi.org/10.24326/ICDSUPL3.A031
Published online: 24 April 2024
ICDSUPL, 3, A031 (2024)
Mutations and polymorphisms in COX1 gene and D-loop region in dogs with solid mammary carcinoma
Kaja Ziółkowska1, Paweł Grychnik1, Krzysztof Kowal1, Angelika Tkaczyk-Wlizło1, Brygida Ślaska1*
1 Institute of Biological Bases of Animal Production, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
* Corresponding author: brygida.slaska@up.lublin.pl
Abstract
In recent years, research on canine cancer has become increasingly popular, with mammary carcinoma being one of the most prevalent forms of the disease in bitches. Mammary carcinoma comes in many different types, including solid mammary carcinoma, which is both highly common (accounting for 11% of all diagnoses) and particularly difficult to treat. Mitochondria play a crucial role in various cellular functions, making even minimal changes in thousands of copies of mtDNA potentially lethal, often resulting in a high mortality rate. The aim of the study was to reveal mutations and polymorphisms in COX1 gene and D-loop region in dogs with solid mammary carcinoma and to determine the relationship of these changes with the process of neoplastic transformation in this type of tumour. Six samples collected during a routine surgical procedure were obtained from tumor tissues, blood, and healthy tissue of two twelve-year-old crossbreed female dogs with diagnosed solid mammary carcinoma with varying degrees of malignancy. DNA extraction from all tissues was performed with a DNeasy Blood & Tissue Kit (Qiagen). DNA samples were assessed qualitatively by electrophoretic separation in 1.5% agarose gel and quantitatively (Nanodrop DeNovix DS-11, Thermo Fisher, Waltham, USA). PCR reactions were carried out using KAPA HiFi PCR Kit reagents (KAPA Biosystems, Wilmington, USA) and two pairs of primers. The results of the study and the bioinformatic analyses of the sequences indicated, a total of 7 polymorphisms and 14 mutations in crossbreed dogs with solid mammary carcinoma of varying degrees of malignancy. Two polymorphisms (m.5444T>C, m.6065A>G) and one mutation (m.16408G>A) were present in both dogs. It is possible that these changes may indicate the process of carcinogenesis. Further research of a more extensive sample of individuals is necessary to validate the relationship between changes in these regions and cancer type or degrees of malignancy.
This research was funded by grant number 2019/35/B/NZ5/00775 from the National Science Centre in Poland.
Keywords: mtDNA, dog, cancer, mutations
How to cite
K. Ziółkowska, P. Grychnik, K. Kowal, A. Tkaczyk-Wlizło, B. Ślaska, 2024. Mutations and polymorphisms in COX1 gene and D-loop region in dogs with solid mammary carcinoma. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.A031