Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: B002
DOI: https://doi.org/10.24326/ICDSUPL3.B002
Published online: 24 April 2024
ICDSUPL, 3, B002 (2024)
The influence of diet with the addition of copper nanoparticles enriched with different sources of dietary fiber on neurometabolism in the rat brain and jejunum
Michalina Pinkosz1*, Magdalena Krauze2, Jerzy Juśkiewicz3, Bartosz Fotschki3, Paulius Matusevičius4, Albert Skórka5, Katarzyna Ognik2
1 Chair and Department of Human Anatomy, Faculty of Medical Sciences, Medical University of Lublin, K. Jaczewskiego 4, 20-090 Lublin, Poland
2 Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy,University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
3 Institute of Animal Reproduction and Food Research, Division of Food Science Polish Academy of Sciences, Juliana Tuwima 10, 10-748 Olsztyn, Poland
4 Department of Animal Nutrition, Veterinary Academy, Lithuanian University of Health Sciences, LT-47181, Kaunas, Lithuania
5 Department of Cardiology, Dr. Tytus Chałubiński Specialist Hospital in Radom, Lekarska 4, 26-610 Radom, Poland
* Corresponding author: pinkoszmichalina00@gmail.com
Abstract
Neurological aging is one of the more important factors responsible for synaptic dysfunction and degradation of neurons. It is a natural consequence of the biochemical pathways in neural cells, promoting oxidation processes, as well as an increase in the amount of free oxygen radicals that damage DNA. Additionally, neurodegeneration can be characterized with disorders in calcium ion homeostasis, abnormal interactions between lipids of cell membranes, formation of neurofibrillary tangles and deposition of toxic protein residues. It has been shown that diet modifications can delay neurometabolic disorders that result from aging, as well as limit the development of neurodegenerative diseases. The aim of this study was to verify the hypothesis regarding the effect of dietary addition of copper nanoparticles (CuNPs; instead of standard CuCO3) in combination with various dietary fibers on changes in biochemical parameters of the hippocampus and jejunum related to metabolic disorders, which appear with neurological aging in rats. Male rats were fed a control diet containing fiber α-cellulose and a standard or enhanced amount of Cu provided as CuCO3 (6.5 or 13 mg/kg diet). The diets of other groups were deprived of CuCO3 and the animals were fed diets supplemented with two CuNPs aforementioned doses, additionally combined with four different types of dietary fiber: α-cellulose (control fiber), pectin, inulin or psyllium (Plantago psyllium). Our study has shown that the addition of dietary CuNPs increased the level of acetylcholinesterase (AChE) and decreased the levels of Tau protein, glycosylated AChE (GAChE) and Ca/calmodulin dependent protein kinase II alpha (CAMK2α) in the examined organs, thus reducing the neurodegenerative changes within the examined tissue. In conclusion, both applied doses of CuNPs, 6.5 mg/kg 13 mg/ kg of a diet, can beneficially modulate certain important parameters describing metabolic and neurodevelopmental status in the hippocampal and small intestinal tissue of rats. With regard to the fiber type added to rats’ diet, the Plantago psyllium fiber strengthened the observed effects of copper nanoparticles. The observed effects should be confirmed in a model study of neurodegeneration.
This work was supported by the National Science Centre in Poland, Grant No. 2021/41/B/NZ9/01104.
Keywords: copper nanoparticles, copper carbonate, dietary fiber, hippocampus, jejunum, metabolic and neurodevelopmental status
How to cite
M. Pinkosz, M. Krauze, J. Juśkiewicz, B. Fotschki, P. Matusevičius, A. Skórka, K. Ognik, 2024. The influence of diet with the addition of copper nanoparticles enriched with different sources of dietary fiber on neurometabolism in the rat brain and jejunum. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.B002