Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: E017
DOI: https://doi.org/10.24326/ICDSUPL3.E017
Published online: 24 April 2024
ICDSUPL, 3, E017 (2024)
Determination of purine adsorption parameters in acetate buffer
M. Szponder, D. Gugała-Fekner
1 Department of Analytical Chemistry, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie Sklodowska University in Lublin, Marii Skłodowska-Curie 3, 20-031 Lublin, Poland
* Corresponding author: michalszponder@outlook.com
Abstract
Research on the kinetics of electrochemical processes and the mechanisms describing these phenomena is currently being conducted intensively. The aim is to provide information regarding the nature of the electric double layer formed due to the adsorption of molecules at the interface of the metal-electrolyte solution and its influence on the rate of electrode processes. The measurements were carried out in a thermostated vessel, in a three-electrode system: a working mercury electrode with a controlled drop surface area (0.009028 cm2) (CGMDE), a saturated KCl silver chloride electrode, and a platinum wire electrode. In the conducted experiments, an electrochemical analyzer µAutolab by Eco Chemie was used, and the measurements were performed at a temperature of 298 K. The basic electrolyte was 0.5 M acetic acid buffer at pH 5. Purine was chosen for the study. The action of biologically active compounds or drugs involves, among other things, disrupting redox processes occurring at the cellular level in every living organism. The interface between phases: electrode – solution can resemble the cell membrane – solution system. Therefore, it becomes necessary to determine the properties of oxidizing-reducing bioprocesses through the evaluation of their electrochemical activity. The applied research techniques allowed for obtaining information about the qualitative state of the electrode surface by determining: the zero charge potential, Ez, and the surface tension, ɤz, at Ez. It has been demonstrated that the differential capacitance curves of the studied systems with the addition of purine do not coincide with the differential capacitance curves of the basic electrolyte– acetic acid buffer. This provides evidence for the adsorption of the tested compound on the mercury electrode throughout the entire range of applied concentrations. The most information about the structure of the double layer and adsorption at the interface of the metal-electrolyte solution is provided by measurements of the double layer capacitance, zero charge potential, and surface tension at this potential.
Keywords: purine, adsorption, elektroreduction
How to cite
M. Szponder, D. Gugała-Fekner, 2024. Determination of purine adsorption parameters in acetate buffer. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.E017