Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: F012
DOI: https://doi.org/10.24326/ICDSUPL3.F012
Published online: 24 April 2024
ICDSUPL, 3, F012 (2024)
Composition and anticancer activity of selected edible mushrooms naturally occurring in Poland
Agata Michalska1*, Małgorzata Sierocka1, Michał Świeca1
1 Department of Biochemistry and Food Chemistry, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland
* Corresponding author: agata.michalska@up.lublin.pl
Abstract
Mushrooms are a wide group of organisms characterized by a wide range of health-promoting properties including anticancer, antioxidant and autoinflammatory. They result from the numerous secondary metabolites (i.a. phenolic compounds and triterpenes) and compounds (polysaccharides or protein/peptides). The research material included selected species of edible mushrooms from Suillus, Lactifluus, Agaricus and Amanita genus. The study included the preparation of active fractions (four-step extraction) and characterization in terms of composition (polyphenols – the Folin-Ciocialteu reagent, triterpenes – the vanillin reagent and polysaccharides – the phenol-sulfur method). The anticancer properties of the obtained fractions were studied in the AGS stomach cancer and HT29 colon cancer lines models according to their ability to inhibit the proliferation process. The highest amount of polysaccharides was determined in the extracts obtained from the weeping milk cap (Lactifluus volemus) (238 mg GluEq/ g d.m.), while the lowest for pepper butter (Suillus variegatus) (1.5 mg GluEq/ g d.m.). Phenolics content ranged from 5.2 mg GAEq/ g d.m. (milkweed (Lactarius deliciosus)) to 12.6 mg GAEq/ g d.m. (pepper butter and weeping milk cap). Importantly, both species of Lactrius contain a high amount of triterpenoids (176 and 237 mg UAEq/ g d.m. for milkweed and weeping milk cap, respectively). The tested extracts inhibited the proliferation of the studied cancer cells. The highest activity against the AGS line (c.a. 86% of growth inhibition) was determined for the extracts obtained from milkweed and both studied toadstool (Amanita rubescens and Amanita fulva). Those extracts as well as those obtained from pepper butter showed also a high antiproliferative activity in the HT29 line model. Surprisingly, both studied Suillus (common butterfly and yellow buttermilk) exhibited only residual activity against the AGS cells. To sum up, extracts from the studied mushrooms are a source of chemicals exhibiting antiproliferative properties. It should be emphasized that the contents of active compounds and activities vary depending on the mushroom species.
The study was supported by the National Science Centre, Poland (NCN) (2022/45/B/NZ9/01892).
Keywords: mushrooms, anticancer properties, triterpenes, phenolics
How to cite
A. Michalska, M. Sierocka, M. Świeca, 2024. Composition and anticancer activity of selected edible mushrooms naturally occurring in Poland. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.F012