Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: H014
DOI: https://doi.org/10.24326/ICDSUPL3.H014
Published online: 24 April 2024
ICDSUPL, 3, H014 (2024)
The voltammetric procedures for determination of acyclovir
Katarzyna Staniec1*, Katarzyna Tyszczuk-Rotko1, Damian Gorylewski1, Aleksy Keller1
1 Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Skłodowska University, Maria Skłodowska-Curie 3, 20-031 Lublin, Poland
* Corresponding author: kasiastaniec2806@gmail.com
Abstract
Antiviral drugs are commonly used in the treatment of many diseases. These drugs help humans and animals reduce the effects of infections caused by viruses. Various groups of antiviral drugs exist due to their mechanism of action, such as reverse transcriptase inhibitors, protease inhibitors, and DNA polymerase inhibitors. Acyclovir (specifically 9‑[(2‑hydroxyethoxy)methyl]guanine, ACV) belongs to the group of DNA polymerase inhibitors. Active form of ACV is acyclovir triphosphate. The mechanism of action of ACV is based on its similarity to one of the nucleosides, deoxyguanosine. The uptake of this compound by the virus blocks the extension of the DNA strand. This is attributed to the lack of the 3’‑hydroxyl group, which blocks the replication of the viral nucleic acid. Due to its low toxicity and high selectivity, ACV remains one of the most commonly used drugs in the treatment of infections caused by viruses such as herpes simplex virus (HSV), varicella-zoster virus (VZV). Many methods for the ACV determination have been described in the literature, e.g., spectroscopy, chromatography and voltammetry. Among these methods, the one with the best limit od determination and cost of analysis is voltammetry. Voltammetry is a method used to determine the content of ACV in pharmaceutical products and biological samples, such as urine and blood serum. This technique allows for measurements using classical three-electrode systems (working electrode, reference electrode and counter electrode), as well as integrated screen-printed sensors (SPEs). Screen-printed electrodes are sensors that are gaining popularity due to their ease of use, cheapness, highly conductive properties as well as good their analytical properties. SPEs can be modified by changing the composition of the ink, e.g. SPEs modified with carbon nanotubes or graphene. The electrode surface can also be modified by applying a metal or polymer film. The miniaturization of measurement systems with SPEs enables their application in portable analyzers. Attempts have been made to characterize and summary of voltammetric methods used to determine ACV on various electrodes.
Keywords: voltammetry, acyclovir, electrochemical sensors
How to cite
K. Staniec, K. Tyszczuk-Rotko, D. Gorylewski, A. Keller, 2024. The voltammetric procedures for determination of acyclovir. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.H014