Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: P016
DOI: https://doi.org/10.24326/ICDSUPL3.P016
Published online: 24 April 2024
ICDSUPL, 3, P016 (2024)
The application of a Pulsed Electric Field on oil seeds
Karolina Sokal1*, Magdalena Kachel-Górecka1
1 Department of Machine Operation and Production Processes Management, Faculty of Production Engineering, University of Life Sciences in Lublin, Głęboka 28, 20-612 Lublin, Poland
* Corresponding author: karolina.sokal@up.lublin.pl
Abstract
The application of Pulsed Electric Field can contribute to an increased germination capability in a shorter time compared to control. Moreover, coating rapeseed seeds with silver nanoparticles (AgNP) or copper nanoparticles (CuNP) could also increase their germination rate. Coating the seeds with nanoparticles might also stimulate the growth of plants stem growth and root length. The aim of the study is to investigate the germination capacity of rapeseed seeds after PEF and nanoparticles application compared to a control sample and their effect on stem and root length. Research hypothesis is that the application of PEF, AgNP and CuNP has a positive effect on rapeseed germination and on plants stem and root length. The method includes soaking the seeds in water and in water with AgNP and CuNP. For analysis were used 200g of seeds and 800ml of water, to which nanoparticles were added at concentrations of 25 and 50ml/l of water. Subsequently, the seeds were subjected to PEF. The variable considered was the amount of supplied energy (kJ/kg) at 1 and 5,5kJ/kg, as well as the quantity and type of AgNP and CuNP. Control tests were conducted in pure water. The germination strength of seeds after applying AgNP and CuNP at concentrations of 25ml/l and 1 and 5,5kJ/kg was for AgNP 88,3; 94,3%, while for CuNP it was 96,0; 95,7%, and for 50ml/l at 1 and 5,5kJ/kg for AgNP 93,3; 94,3%, and for CuNP 93,3; 94,3%. In the case of seeds soaked in AgNP, a significant increase in stem length was observed compared to the control, both for concentrations of 25ml/l at 1 and 5,5kJ/kg (36,9; 46,7%), and 50ml/l at 1 and 5,5kJ/kg (18,9; 43,8%). After applying CuNP, a significant increase was observed for both concentrations of 25 ml/l at 1 and 5,5kJ/kg (48,4; 36,2%), as well as 50ml/l at 1kJ/kg (34,4%), while a decrease of 23,8% was noted for 5,5kJ/kg. After soaking in AgNP, a significant increase in root elongation was observed compared to the control sample, both for concentrations of 25ml/l at 1 and 5,5kJ/kg (26,1; 71,2%), and (60,2; 103,0%) for 50ml/l. Soaking the seeds in CuNP resulted in a reduction in root elongation for 25ml/l at 1kJ/kg by 41,0%, and for 50ml/l at 1kJ/kg by 4,3%. However, the increase occurred at the concentration of 25ml/l at 5,5kJ/kg by 72,0% and 50ml/l at 5,5kJ/kg by 3,0%. In summary, the application of PEF with AgNP and CuNP does not lead to a significant increase in germination capability, but it does positively affect the elongation of rapeseed roots.
Keywords: Pulsed Electric Field, nanoparticles, rapeseed seeds, germination
How to cite
K. Sokal, M. Kachel-Górecka, 2024. The application of a Pulsed Electric Field on oil seeds. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.P016