Volume: 4, 2025
4th International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A015
DOI: https://doi.org/10.24326/ICDSUPL4.A015
Published online: 9 April 2025
ICDSUPL, 4, A015 (2025)
Neuronostatin modulates isoproterenol-induced lipolysis in white adipocytes
Małgorzata Krążek1*, Joanna Fiedorowicz1, Tatiana Wojciechowicz1, Joanna Grześkowiak1, Marek Skrzypski1
1 Department of Animal Physiology, Biochemistry and Biostructure, Poznan University of Life Sciences, Wołyńska 35, 60-637 Poznań, Poland
* Corresponding author: malgorzata.krazek@up.poznan.pl
Abstract
Obesity is a growing global health problem, contributing to metabolic disorders such as type 2 diabetes, cardiovascular diseases, and insulin resistance. Understanding the mechanisms regulating lipid metabolism is crucial for developing potential therapeutic targets. Neuronostatin (NST) is a peptide hormone encoded by the somatostatin gene. Its activity is mediated by the GPR107 receptor. Previous studies have shown that NST plays a role in energy homeostasis by modulating appetite, gastrointestinal transit, and glucagon secretion. Additionally, NST regulates white and brown adipogenesis in rodents. However, the effect of NST on mature white fat cells and lipid metabolism remains unknown. This study aims to investigate the influence of NST on basal and isoproterenol-induced lipolysis in 3T3-L1 adipocytes. The differentiated 3T3-L1 adipocytes cultured in DMEM High Glucose medium were incubated with isoproterenol and NST for 3 or 24 hours. After incubation, the culture media were collected, and lipolysis was evaluated by measuring glycerol using colorimetric assays. The levels of glycerol were normalized to the total protein content. Gene expression of key lipolytic regulators was analyzed by qPCR. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post hoc test, or the Kruskal-Wallis test when normality assumptions were not met. NST treatment reduced isoproterenol-induced lipolysis, as evidenced by decreased glycerol release after 24 hours. By contrast, NST had no effect on basal glycerol release. Furthermore, in the presence of isoproterenol, NST downregulated the expression of hormone-sensitive lipase (HSL), adipose triglyceride lipase (ATGL), and protein kinase A (PKA). Neuronostatin attenuates isoproterenol-induced lipolysis in white adipocytes. These findings suggest a novel regulatory role of NST in lipid metabolism and its potential impact on energy balance and metabolic regulation.
Keywords: neuronostatin, lipolysis, adipocytes, metabolism
How to cite
M. Krążek, J. Fiedorowicz, T. Wojciechowicz, J. Grześkowiak, M. Skrzypski, 2025. Neuronostatin modulates isoproterenol-induced lipolysis in white adipocytes. In: 4th International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL4.A015