Volume: 4, 2025
4th International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A018
DOI: https://doi.org/10.24326/ICDSUPL4.A018
Published online: 9 April 2025
ICDSUPL, 4, A018 (2025)
Does the effect of copper nanoparticles on DNA methylation and the redox status of rats depend on the diverse physiological functions of dietary fiber?
Aleksandra Marzec1*, Ewelina Cholewińska1, Bartosz Fotschki2, Jerzy Juśkiewicz2, Katarzyna Ognik1
1 Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
2 Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10–748 Olsztyn, Poland
* Corresponding author: aleksandra.marzec@up.lublin.pl
Abstract
Nanotechnology is increasingly used in the food and medical industries, but there is still a need to understand the mechanisms of nanoparticle absorption and their potential impact on the body. The aim of this study was to determine how different forms of dietary fiber modulate copper nanoparticle absorption and its consequences on the immune and redox state. Healthy outbred male Wistar rats 6-wk aged (Cmdb:Wi) was fed for 6 weeks standard semi-purified rat diet with dietary addition of two Cu-NP dosages (recommended and two times higher; 6.5 and 13 mg/kg diet) and combined with different types of dietary fiber; 6% of a diet (control cellulose, inulin with prebiotic effect, psyllium with bulk effect, pectin with viscous effect; 10 groups, n=10 per group). The digestibility and utilization tests (balance tests) of Cu was carried out. At the end of the experiment, the rats was fasted for 12 h and anaesthetized. After blood collection and euthanasia, the small intestine, caecum, colon, liver, thoracic arteries and chosen internal organs (heart, kidneys, brain, testes, femur, lungs, spleen, thigh muscle) was dissected and weighed. Blood samples were collected from the caudal vena cava into heparinized tubes and EDTA tubes, and finally the rats were euthanized by cervical dislocation. After that the heart, lungs, jejunum, liver, pancreas, kidneys, spleen and tests were dissected. Samples of the collected organs were used to prepare tissue homogenates. The levels of selected redox status indicators – SOD, CAT, Cp, TAS, MDA, 3-NT, PC, 8-OHdG, DNA repair enzymes – APE-1 and OGG1, and markers of apoptotic cell death – Casp3 and Casp8 were determined in the blood plasma. The activity of antioxidant enzymes – SOD and CAT and the level of lipid peroxidation marker – MDA in tissue homogenates of heart, lungs, jejunum, liver, pancreas, kidneys, spleen and testes were determined. The obtained results indicate that the type of fiber significantly affects the bioavailability of copper nanoparticles, with soluble fiber fractions promoting its higher absorption, while insoluble fiber fractions limited this process. Changes in the levels of oxidative stress markers and cytokines suggest that excessive absorption of copper nanoparticles may lead to disturbances in oxidative homeostasis and inflammatory response. These results emphasize the important role of fiber in regulating copper nanoparticle absorption and its effects on the body.
This work was supported by the National Science Centre in Poland, Grant No. 2021/41/B/NZ9/01104
Keywords: nanoparticles, dietary fiber, oxidative stress, immunomodulation, redox
How to cite
A. Marzec, E. Cholewińska, B. Fotschki, J. Juśkiewicz, K. Ognik, 2025. Does the effect of copper nanoparticles on DNA methylation and the redox status of rats depend on the diverse physiological functions of dietary fiber?. In: 4th International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL4.A018