Volume: 4, 2025
4th International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: P011
DOI: https://doi.org/10.24326/ICDSUPL4.P011
Published online: 9 April 2025
ICDSUPL, 4, P011 (2025)
Creation of AtPRN2:citrine and AtPRN2:3xcherry transgenic lines to study secondary wood in A. thaliana
Zuzanna Majewska1*, Katarzyna Sokołowska1, Alicja Dołzbłasz1
1 Department of Plant Developmental Biology, University of Wroclaw, Kanonia 6/8, Wrocław 50-328, Poland
* Corresponding author: majewskazuz@o2.pl
Abstract
During secondary growth that ensures progressive thickening of plant axes, secondary conductive tissues are produced. Secondary xylem (wood) is characterised by heterogenous character, as it is composed of both dead and living elements. Arabidopsis thaliana is a particulary valuable model plant for molecular oriented research, and importantly, its hypocotyl serves as an excellent model in wood research. Bearing in mind difficulties in studying trees, in our wood oriented research we use Arabidopsis thaliana. Using standard procedures of molecular cloning and transformation with the “floral dip” method we created Arabidopsis transgenic reporter lines to fluorescently visualise living parenchyma cells in secondary wood. Selection was performed in long day conditions of growth (LD, 16h day and 8h night), and was based on the analysis of plants phenotype and fluorescence signal strength (and pattern of expression). We picked the best AtPRN2:citrine and AtPRN2:3xcherry lines for our further secondary wood related studies. The pattern of expression was then validated in short day conditions of growth (SD, 16h night and 8h day), when Arabidopsis plants form secondary wood in their hypocotyls. Our AtPRN2:3xcherry line proved to be specific for living parenchyma cells neighboring vessel elements. Known from literature pattern of AtPRN2 promoter activity localisation in parenchyma and fibers is probably the result of marker protein (GUS) migration. Our AtPRN2:citrine line (free citrine protein) also has such a broader localisation of the signal. The goal of further studies is to use the reporter line(s) to study whether the number and localisation of living parenchyma cells neighboring vessel element change in Arabidopsis mutant plants with modified structure of secondary wood of the hypocotyl. Our new lines open up new methodological prospects in studying secondary wood.
Funding: BPIDUB.4610.174.2022 to A.D.
Keywords: Arabidopsis thaliana, fluorescence reporter lines, secondary wood
How to cite
Z. Majewska, K. Sokołowska, A. Dołzbłasz, 2025. Creation of AtPRN2:citrine and AtPRN2:3xcherry transgenic lines to study secondary wood in A. thaliana. In: 4th International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL4.P011