Volume: 5, 2026
5th International PhD Students’ Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A014
DOI: https://doi.org/10.24326/ICDSUPL5.A014
Published online: 22 April 2026
Effect of Lisosan G supplementation on oxidative status in a rat model of hepatocellular carcinoma
Maja Gronowska*¹, Bartłomiej Szymczak² and Marta Wójcik3
1 Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 30 Głęboka St., 20-612 Lublin, Poland
2 Sub-Department of Pathophysiology, Department of Preclinical of Veterinary Sciences, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 12 Akademicka St., 20-033 Lublin, Poland
3 Oncology Lab. Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 30 Głęboka St., 20-612 Lublin, Poland
* Corresponding author: maja.gronowska@up.edu.pl
Oxidative stress is a major factor involved in the pathogenesis of liver diseases, including hepatocellular carcinoma (HCC). Excessive production of reactive oxygen species (ROS) and impaired antioxidant defence contribute to cellular damage, lipid peroxidation and disruption of enzymatic homeostasis.
The aim of this study was to investigate the effect of Lisosan G (LG) supplementation on antioxidant status and oxidative stress markers in rat liver under experimental HCC conditions. The study included two main groups: a control group and a group with experimentally induced HCC. Within each group, animals were further divided into three subgroups receiving a standard diet, a diet supplemented with 2.5% LG, or a diet supplemented with 5% LG. Liver samples were collected for biochemical analysis.
Subcellular fractions were isolated using differential ultracentrifugation. The activity of heme oxygenase-1 (HO-1), DT-diaphorase and catalase was measured spectrophotometrically. The level of malondialdehyde (MDA) was determined as an indicator of lipid peroxidation.
HCC induction resulted in a marked increase in oxidative stress, demonstrated by elevated MDA levels, increased activity of HO-1 and catalase, and reduced DT-diaphorase activity. Administration of Lisosan G attenuated oxidative damage, as evidenced by decreased MDA levels, and enhanced antioxidant defence mechanisms through upregulation of HO-1 and catalase, along with partial recovery of DT-diaphorase activity.
Overall, the results suggest that Lisosan G exerts a modulatory effect on redox homeostasis in the liver and may support antioxidant defence under conditions of hepatocellular injury, indicating its potential role as an adjunct in HCC-related oxidative stress.
Keywords: antioxidant enzymes; hepatocellular carcinoma; Lisosan G
How to cite
Gronowska M., Szymczak B., Wójcik M., 2026. Effect of Lisosan G supplementation on oxidative status in a rat model of hepatocellular carcinoma. In: 5th International PhD Students’ Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL5.A014