Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A001
DOI: https://doi.org/10.24326/ICDSUPL2.A001
Published online: 19 April 2023
ICDSUPL, 2, A001 (2023)
Changes in the concentrations of proteins, glucoses and triglycerides in fat bodies of honeybees fed rapeseed and phacelia pollens
Maciej Sylwester Bryś1*, Aneta Strachecka1
1 Department of Invertebrate Ecophysiology and Experimental Biology, University of Life Sciences in Lublin, Doświadczalna 50a, 20-280 Lublin, Poland
* Corresponding author: maciej.brys@up.lublin.pl
Abstract
The subcuticular fat body of bees has pleomorphic structure of the cells and a segmental character. Each segment has its own distinctive function, the combination of which regulates the joint metabolism in bees. Such a character of the fat body is determined by the quality and volume of food during preimaginary development and in imago, especially in the first days of life. One of the problems of modern beekeeping is malnutrition of bees resulting from the lack of sufficient food and its lack of balance. To counteract this situation, bees are fed by beekeepers. That is why it is so important to develop an optimal diet for these pollinators. Determining the properties of individual pollen as the basic component of the bee diet is the first step in developing such an optimal food. The aim of the study was to determine the effect of pollen monodiet, on the example of rapeseed and phacelia pollen, on changes in the concentrations of protein, glucose and triglycerides in some segments of the honey bee’s fat body. One-day‑old workers were divided into three groups, which were fed: I group – the sugar candy; II group – sugar candy with the addition of rapeseed pollen; III group – candy with the addition of phacelia pollen. On days 7 and 14, bees were collected from each group and sternite, tergite 3 and tergite 5 were isolated from them. These tissues were homogenized in 0.6% NaCl and frozen. Then, the biochemical parameters were determined with commercial kits. Even a small addition of pollen increased the concentrations of proteins, glucose and triglycerides in all segments of the fat body of 7 and 14‑day‑old bees compared to the control group. The glucose concentrations were higher in the fat body of 7 and 14-day‑old workers fed with phacelia than with rapeseed. The protein concentrations were always higher in bees fed rapeseed compared to other groups. The triglyceride concentrations were higher in the fat body of: tergite 3 in bees fed rapeseed pollen, and tergite 5 in bees fed with phacelia pollen. Each of the pollen affects the concentrations of biochemical parameters in the fat body to a different extent. It can therefore be concluded that only the composition of various pollens will affect the regulation and stabilization of metabolism.
Continuation of research is planned as part of the research project “PRELUDIUM-21”, No. 2022/45/N/NZ9/01333, entitled “Aging-related changes in the systems involved in bee defence responses in the context of the pollen monodiet as a key environmental stressor”.
How to cite
M.S. Bryś, A. Strachecka, 2023. Changes in the concentrations of proteins, glucoses and triglycerides in fat bodies of honeybees fed rapeseed and phacelia pollens. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A001