ICDSUPL2-A025

Volume: 2, 2023
2^nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT

Abstract number: A025

DOI: https://doi.org/10.24326/ICDSUPL2.A025

Published online: 19 April 2023

ICDSUPL, 2, A025 (2023)

Effect of feeding diet containing coccidiostat and administration of antibiotics to turkeys in the first days after hatching on redox status in blood serum

Radosław Smagieł¹*, Anna Stępniowska¹, Dariusz Mikulski², Jan Jankowski², Katarzyna Ognik¹, Paulius Matusevicius³

¹ Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland

² Department of Poultry Science and Apiculture, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland

³ Department of Animal Nutrition, Lithuanian University of Health Sciences, Tilzes str. 18, LT47181, Kaunas, Lithuania

^{* Corresponding author: radoslaw.smagiel@up.lublin.pl}

Abstract

The experiment verified the hypothesis that early administration of an antibiotic, especially when fed a diet containing a coccidiostat, may increase oxidative reactions and impair antioxidant defense. The experiment was conducted on 1152 one-day-old turkeys divided into 6 treatments: CM(-), EM(-), DM(-), CM(+), EM(+), DM(+). Turkeys in the M(-) groups did not receive monensin in the diet, while those in the M(+) groups received monensin at a dose of 90 mg/kg of feed. Turkeys in groups C, received no antibiotic supplement (control groups), while those in groups E received enrofloxacin at a dose of 10 mg/kg BW for the first 5 days of life, and those in groups D received doxycycline at a dose of 50 mg/kg BW for the first 5 days of life. The birds were vaccinated against APV and NDV on day 1 of life and against ORT on day 28 of life. On days 7 and 56, blood was collected from 8 birds in the group to evaluate MDA, AOPP, 8-isoprostane, TAS levels, and CAT activity. The study showed that feeding a diet containing a coccidiostat without or with the addition of doxycycline or enrofloxacin did not indicate lipid oxidation measured by the level of MDA. However, the measurement of a more sensitive indicator, ie 8-isoprostanes, showed an increase in the level of this indicator under the influence of antibiotic therapy. Early administration of doxycycline resulted in an unfavorable reaction consisting in an increase in protein and fatty acid oxidation products, and this effect was visible immediately after antibiotic treatment and lasted until 56 days of age. Early administration of doxycycline and simultaneous feeding with a diet supplemented with monensin additionally intensified the adverse effect of oxidation of the above-mentioned molecules. Similarly, early administration of enrofloxacin, especially when turkeys were fed a diet supplemented with monensin, increased the level of AOPP and 8-isoprostanes, but in a period quite distant from the administration of enrofloxacin, as only at 56 days of age. Under the influence of early administration of doxycycline and enrofloxacin, a decrease in the total antioxidant potential of TAS and an increase in CAT activity were found, and administration of these antibiotics together with monensin intensified this effect. The conducted research clearly shows that early administration of antibiotics to turkeys induces unfavorable oxidation reactions in cells and worsens antioxidant defense. The adverse effect is exacerbated by concomitant use of a diet supplemented with monensin and early use of doxycycline or enrofloxacin.

This work was supported by the National Science Centre in Poland, Grant No. 2020/39/B/NZ9/00765.

How to cite

R. Smagieł, A. Stępniowska, D. Mikulski, J. Jankowski, K. Ognik, P. Matusevicius, 2023. Effect of feeding diet containing coccidiostat and administration of antibiotics to turkeys in the first days after hatching on redox status in blood serum. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A025