Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A027
DOI: https://doi.org/10.24326/ICDSUPL2.A027
Published online: 19 April 2023
ICDSUPL, 2, A027 (2023)
Effect of feeding diet containing coccidiostat and antibiotics administration to turkeys on gut-brain axis interplay and possible animal welfare consequences
Przemysław Sołek1*, Radosław Smagieł1, Bartosz Kwiatkowski2, Jan Jankowski3, Katarzyna Ognik1
1 Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
2 Dr. Tytus Chałubiński Specialist Hospital in Radom, Department of Cardiology, Lekarska 4, 26-610 Radom, Poland
3 Department of Poultry Science and Apiculture, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland
* Corresponding author: przemyslaw.solek@up.lublin.pl
Abstract
The experiment verified the hypothesis that early administration of an antibiotic, especially when fed a diet containing a coccidiostat, may regulate the microbiota-gut-brain axis. The experiment was conducted on one-day-old turkeys divided into 4 treatment groups: C, E, D, M. Turkeys in groups C, received no antibiotic supplement (control group), while those in group E received enrofloxacin at a dose of 10 mg/kg BW and those in group D received doxycycline at a dose of 50 mg/kg BW for the first 5 days of life while those in the M group received monensin at a dose of 90 mg/kg of feed. The birds were vaccinated against APV and NDV on day 1 of life. On day 7 the hippocampus were collected from 8 birds per group to evaluate the intracellular signalling pathway related to neural synaptic plasticity. The study showed that feeding a diet containing a coccidiostat without or with the addition of doxycycline or enrofloxacin can upregulate the BDNF and CDNF neurotrophic factors. However, effects were noted only for E and M but not for D groups. This was an important observation because BDNF and other neurotrophins protect neurons from death and also induce neurogenesis and synaptic plasticity during homeostatic regulation. Moreover, E and M substances administration activated Erk1/2 and Akt/mTOR signaling pathways. In turn, BDNF affected CREB and reduced eEF2/Akt phosphorylation, while impaired Akt-dependent signaling pathway affects neuronal communication and neuromodulation. In addition, reduced but not eliminated activation of the Akt/mTOR/eEF2 pathway plays a key role in response to stress conditions, including changes in large intestine function. We previously proved that doxycycline administration increased cortisol and decreased serotonin levels in plasma. This may explain the downregulation of BDNF and CDNF and may additionally be related to selective signaling inhibition via CaMKI kinase. Thus, it seems that overlapping effects of synaptic activity and gut-brain axis activation by the microbiome may contribute to mood improvement. Taken together, our results highlight the essential role of the BDNF/CaMKI/CREB signaling pathway in neuronal activation, synaptic plasticity and CNS disorders.
This work was supported by the National Science Centre in Poland, Grant No. 2020/39/B/NZ9/00765.
How to cite
P. Sołek, R. Smagieł, B. Kwiatkowski, J. Jankowski, K. Ognik, 2023. Effect of feeding diet containing coccidiostat and antibiotics administration to turkeys on gut-brain axis interplay and possible animal welfare consequences. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A027