Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A036
DOI: https://doi.org/10.24326/ICDSUPL2.A036
Published online: 19 April 2023
ICDSUPL, 2, A036 (2023)
Influence of glucose content on the proliferation of satellite cells
Karolina Zygmunt1*, Wojciech Witarski1, Katarzyna Piórkowska1
1 Department of Animal Molecular Biology, National Research Institute of Animal Production, street Krakowska 1, 32-083, Balice near Krakow, Poland
* Corresponding author: karolina.zygmunt@iz.edu.pl
Abstract
The potential of satellite cells, being precursors of skeletal muscles, can be used in in vitro meat production technology. The high level of satellite cell proliferation is crucial in recreating muscle structure in vitro and is required to provide mitogenic factors and other essential components like amino acids, vitamins, and glucose – being the source of energy for proliferating cells. The literature data presented on low and high glucose approaches in cell culture medium is inconclusive. The study aimed to compare the effect of the culture medium on the proliferation rate of bovine satellite cells with low (1 g/L) and high (4.5 g/L) glucose content (DMEM supplemented with 20% FBS and antibiotic Primocin). The proliferation rate based on the CellTiterBlue (Promega) viability test on the third and fifth days of culture was examined. Afterwards, the expression of genes involved in myogenesis – Pax7 and Myogenic Regulator Factors (Myf5, MyoD, Myf6, and MyoG) were reviewed on the third and fifth day of incubation by qPCR analysis. A statistically higher proliferation rate of cells was observed on the third day in a medium supplemented with 4.5 g/L of glucose (p < 0.05). However, no statistically significant differences in proliferation rate were observed between groups with various glucose content on the fifth day. Statistically higher relative expression levels of Pax7 and Myf5 genes were observed in the group supplemented with 1 g/L glucose on the third day, compared with the group supplemented with 4.5 g/L (p<0.05). No statistically significant differences in relative gene expression were observed between groups on the fifth day. The obtained results indicate that the lower glucose content in the medium, at the beginning of proliferation, ensures a lower rate of differentiation – through the expressing Pax7 and Myf5 genes, which are crucial in the self-renewal process of the satellite cell pool. However, conducted research shows that lower glucose content does not ensure a higher rate of proliferation.
This study has been supported by National Science Center, Miniatura 5 – grant, number 2021/05/X/NZ9/01365.
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How to cite
K. Zygmunt, W. Witarski, K. Piórkowska, 2023. Influence of glucose content on the proliferation of satellite cells. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.A036