Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: P008
DOI: https://doi.org/10.24326/ICDSUPL2.P008
Published online: 19 April 2023
ICDSUPL, 2, P008 (2023)
Microscopy based evaluation of oat resistance to powdery mildew
Magdalena Cieplak1*, Sylwia Okoń1, Aleksandra Nucia1, Aisling Reilly2
1 Institute of Plant Genetics, Breeding and Biotechnology, University of Life Science in Lublin, Faculty of Agrobioengineering, University of Life Science in Lublin, Akademicka 15, 20-950 Lublin, Poland
* Corresponding author: magdalena.cieplak@up.lublin.pl
Abstract
Blumeria graminis f. sp. avenae (Bga) is the causal agent of powdery mildew in oats (Avena sativa L.) a crucial foliar disease that can cause yield losses of around 10% up to 39%. The introduction of oat cultivars that carry effective resistance genes is the most effective, economical, and environmentally friendly method of infection control. Identification of the resistance sources and characterization of underlying defense mechanisms will be useful for future breeding programs. It also could be used in more advanced cellular and molecular studies to explore the genetic basis of resistance in oat-powdery mildew interactions. The purpose of this study was to assess and characterized powdery mildew resistance in the genotypes with known resistance genes (Pm4, Pm7, and Pm11) with a moderate and high level of resistance in comparison to susceptible cultivars. In this research, we also the present process of infection pathogens in host tissues. The process of infection and resistance response of each gene was characterized by scoring papillae formation, haustorial and hyphe encasements and programmed cell death (PCD) using light and fluorescence microscopy. The results confirmed differences in process of infection and response between genotypes with different Pm genes. The experiment approved that haustorial and hyphe encasement and programmed cell death play a key role in resistance response in oat. In genotypes with Pm4 and Pm7 was observed more cell death than in genotypes Pm11 in which a higher amount of haustorial encasements was found. This study paves the way for future studies on oat’s resistance response mechanism.
How to cite
M. Cieplak, S. Okoń, A. Nucia, A. Reilly, 2023. Microscopy based evaluation of oat resistance to powdery mildew. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.P008