Volume: 2, 2023
2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: P011
DOI: https://doi.org/10.24326/ICDSUPL2.P011
Published online: 19 April 2023
ICDSUPL, 2, P011 (2023)
Comparizon of the effects of biostimulant and several growth regulators on biochemical changes during ex vitro acclimatization of microcuttings in Echinacea purpurea Moench. ‘Raspberry Truffle’
Monika Grzelak1*, Andrzej Pacholczak1, Karolina Nowakowska1
1 Section of Ornamental Plants, Faculty of Horticulture, Warsaw University of Life Sciences; Nowoursynowska 166 st., 02-787 Warsaw, Poland
* Corresponding author: monika_grzelak@sggw.edu.pl
Abstract
Micropropagation allows to rapidly obtain high numbers of high quality plants. However, during the last stage of the procedure, i.e. acclimatization a high mortality rate may occur. This is due to transfer of plantlets from sterile laboratory conditions to the exterior environment where the conditions are stressful for young non adapted plants. Stress during acclimation may be minimized by using growth regulators or biostimulators which improve a general plant condition. The aim of this work was to develop a protocol of acclimation ex vitro of microcuttings of Echinacea purpurea Moench. ‘Raspberry Truffle’. The following preparations/substances were used: water solution of biostimulator Goteo (0.3%) and growth regulators: abscisic acid (ABA 0.001%), brassinolide (BR 0.001%) and epibrassinolide (BL 0.001%). The experimental material came from the in vitro cultured plants. Rooted microcuttings were acclimated in plastic boxes filled with the mixture of peat and perlite (2:1, v/v), pH 5.0-6.0, placed in a phytotrone at 25oC under 16 h day (50 μmol·m-2·s-1). After 6 weeks plantlets were transferred to the greenhouse. There were 5 treatments, each in triplicate, each containing 10 microcuttings. Plant material (0.25 g samples) was collected at the beginning of experiment (1st term), at the end of acclimation in phytotrone (2nd term) and in the greenhouse (3rd term). The effect of acclimation and the treatments on chlorophyll, carotenoids, hydrogen peroxide, soluble proteins content and catalase activity were measured. Within acclimation the content of chlorophyll carotenoids, soluble protein and catalase activity increased. Hydrogen peroxide content was the highest at the 2nd term. Moreover it is suggested that under the influence of these preparations, there were changes in above mentioned parameters in plants.
How to cite
M. Grzelak, A. Pacholczak, K. Nowakowska, 2023. Comparizon of the effects of biostimulant and several growth regulators on biochemical changes during ex vitro acclimatization of microcuttings in Echinacea purpurea Moench. ‘Raspberry Truffle’. In: 2nd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL2.P011