Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A003
DOI: https://doi.org/10.24326/ICDSUPL3.A003
Published online: 24 April 2024
ICDSUPL, 3, A003 (2024)
Proteomic and molecular detection of antibiotic resistant bacteria from wounds of companion animals
Klaudia Bulanda1*, Anna Lenart-Boroń2, Klaudia Stankiewicz2
1 Department of Forest Ecosystems Protection, Faculty of Forestry, University of Agriculture in Krakow, 29 Listopada Ave. 46, 31-425 Krakow, Poland
2 Department of Microbiology and Biomonitoring, Faculty of Agriculture and Economics, University of Agriculture in Krakow, Adam Mickiewicz Ave. 24/28, 30-059 Krakow, Poland
* Corresponding author: klaudia.bulanda@student.urk.edu.pl
Abstract
Keeping skin wounds clean in small animals is a big challenge, which is why they often become infected. This creates a risk of infection with antibiotic-resistant bacteria, which may then be transmitted to the owner, or the antibiotic resistance gene may be transferred to the owner’s microbiota. Most bacterial pathogens found in small animals are also commonly found in humans, which is a potential threat of infection for anyone in contact with a sick animal. The paucity of data on this topic was the reason it was undertaken in the study below. For this purpose, 145 swabs were taken from animal wounds and inoculated on selective media to isolate and identify bacteria. Additionally, species identification of isolated microorganisms was performed using MALDI-TOF. Then, drug susceptibility tests were performed using the disk diffusion method, and the occurrence of genes responsible for bacterial resistance to various groups of antimicrobial agents was assessed using the PCR technique. The obtained results were subjected to statistical analysis using the chi-square test. The most common bacteria were classified as Staphylococcus spp. (n=37, 27.21%), Enterococcus spp. (n = 17, 12.50%) and Escherichia (n = 11, 8.09%). Among the group of Enterobacterales, the resistance to amoxicillin/clavulanic acid was the most prevalent (detected in 68.3% of strains). All strains of Pseudomonas spp. (n=7) were resistant to four of eight tested antibiotics. Finally, Acinetobacter (n=9) showed the highest percentage of resistance to tylosin (55.5% of strains). In the case of Gram-positive bacteria, none of the examined antimicrobials were effective against all Enterococcus spp. strains (n = 15). Staphylococcus spp. was most frequently resistant to clindamycin (39.2% of strains). In PCR tests, ten of the 12 tested genetic determinants of antimicrobial resistance were identified in the isolated bacteria. Another disturbing observation resulting from the conducted research is the detection of bacteria that are also human pathogens, which were resistant to almost all or all of the tested antimicrobials and had three or four antibiotic resistance genes. Given the risk of transfer of resistant bacteria between animals and humans, the results obtained are disturbing, and the increasing demand for advanced therapies in companion animals may necessitate the search for new antimicrobial therapies in veterinary medicine in the future.
This research was funded by the measures of the state budget Polish Ministry of Education and Science under the program “Student scientific circles create innovations” – Research on the effectiveness of innovative hyaluron bionanocomposites with nanoencapsulated ozonated olive oil, graphene, and nanosilver against bacteria that infect wounds in dogs and cats (Agreement No. SKN/SP/569551/2023).
Keywords: antibiotic resistant bacteria, companion animals, antibiotic resistance genes, veterinary medicine, wound infections
How to cite
K. Bulanda, A. Lenart-Boroń, K. Stankiewicz, 2024. Proteomic and molecular detection of antibiotic resistant bacteria from wounds of companion animals. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.A003