Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A019
DOI: https://doi.org/10.24326/ICDSUPL3.A019
Published online: 24 April 2024
ICDSUPL, 3, A019 (2024)
Are the biodistribution and metabolic effects of copper nanoparticles dependent on differences in the physiological functions of dietary fibre?
Aleksandra Marzec1*, Jerzy Juśkiewicz2, Rafał Płatos3, Wojciech Dworzański3, Katarzyna Ognik1
1 Department of Biochemistry and Toxicology, Faculty of Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
2 Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10–748 Olsztyn, Poland
3 Department of Cardiology, Dr. Tytus Chałubiński Specialist Hospital in Radom, Lekarska 4, 26-610 Radom, Poland
* Corresponding author: aleksandra.marzec@up.lublin.pl
Abstract
The aim of the study was to verify this hypothesis by assessing the effect of the inclusion of CuNPs in the diet of rats at the recommended level (6.5 mg Cu/kg diet) or double that level (13.0 mg Cu/kg diet) in combination with various sources of dietary fibre – cellulose, pectin, inulin or psyllium – on the biodistribution of Cu, by analyzing the intake, excretion, digestibility, utilization, and distribution of Cu in the tissues. Rats was fed for 6 weeks standard diet with dietary addition of two Cu-NP dosages and combined with different types of dietary fibre. Groups C and CH were fed a control diet with standard and enhanced Cu content in the mineral mixture (6.5 and 13 mg/kg from CuCO3, respectively) with 8% of cellulose as dietary fibre source; groups CN and CNH were fed diets with supplementation of Cu-NP (6.5 and 13 mg/kg from Cu-nanoparticles, respectively) with 8% of cellulose dietary fibre source; groups PN and PNH were fed diets with supplementation of Cu-NP (6.5 and 13 mg/kg from Cu-nanoparticles, respectively) with 2% of cellulose and 6% of pectin dietary fibre source; groups JN and JNH were fed diets with supplementation of Cu-NP (6.5 and 13 mg/kg from Cu-nanoparticles, respectively) with 2% of cellulose and 6% of inulin dietary fibre source; groups SN and SNH were fed diets with supplementation of Cu-NP (6.5 and 13 mg/kg from Cu-nanoparticles, respectively) with 2% of cellulose and 6% of psyllium dietary fibre source. Blood was collected from 10 rats from the group for the determination of hematological parameters. Replacing CuCO3 in the diet with CuNPs had a positive effect on the biodistribution of Cu in the body by reducing its excretion, improving its digestibility and utilization, and limiting its accumulation in the brain and muscles. Increasing the level of CuNPs in the diet increased total Cu intake. The addition of pectin, inulin and psyllium to the high CuNPs diet significantly increased Cu excretion without negatively affecting its digestibility and utilization, and prevented its excessive accumulation in the brain and muscles of rats, especially in the case of inulin. The results suggest that the addition of dietary fiber to the diet of rats ensures fiber homeostasis in the event of excessive CuNPs consumption by modifying Cu bioavailability.
This work was supported by the National Science Centre in Poland, Grant No. 2021/41/B/NZ9/01104.
Keywords: biodistribution, copper, dietetary fibre
How to cite
A. Marzec, J. Juśkiewicz, R. Płatos, W. Dworzański, K. Ognik, 2024. Are the biodistribution and metabolic effects of copper nanoparticles dependent on differences in the physiological functions of dietary fibre?. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.A019