Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A023
DOI: https://doi.org/10.24326/ICDSUPL3.A023
Published online: 24 April 2024
ICDSUPL, 3, A023 (2024)
The use of rapid diagnostic immunochromatographic tests and PCR method in the detection of the presence of vaccine viruses (CPV) in feces in vaccinated dogs
Maria Pisarek1*
1 Department of Epizoology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine of the University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland
* Corresponding author: maria.pisarek@up.lublin.pl
Abstract
Canine parvovirus (CPV) is widespread all over the world. Dogs of all ages that do not have protective equipment are susceptible to the infection the level of antibodies against the virus. The disease is most severe reported in puppies from approx. 6 weeks to 4–6 month of life. The mortality rate of untreated animals is very high high and is up to approximately 90%. Parvovirus should be considered whenever it occurs gastrointestinal symptoms, especially in young, unvaccinated people dogs. Hematological examination can be demonstrated in sick individuals leukopenia, very often develop coagulation disorders. The most common laboratory method used to detect the virus in stools is the polymerase chain reaction, especially real-time PCR. In the diagnosis of the disease a hemagglutination inhibition test and examination may also be used feces for the presence of CPV antigen at using rapid immunochromatographic tests. The aim of the study was to determine whether the rapid diagnostic CPV Ag (Vet Expert) tests are able to detect the presence of vaccine viruses in feces collected from dogs vaccinated against parvovirosis. The tested dogs were administered vaccines once, and then feces and conjunctival sac swabs were collected from all animals on the 2nd, 5th, 7th, 10th, 14th, 20th and 25th day after vaccination for molecular tests in order to perform rapid immunochromatographic tests in for the detection of CPV antigens. To summarize the results of our own research, it should be stated that rapid immunochromatographic tests do not are capable of detecting CPV vaccine viruses. However, they are an excellent tool to detect these pathogens in infected animals field strains of viruses and those revealing clinical symptoms of the disease.
Keywords: dogs, CPV, PCR, immunochromatographic tests
How to cite
M. Pisarek, 2024. The use of rapid diagnostic immunochromatographic tests and PCR method in the detection of the presence of vaccine viruses (CPV) in feces in vaccinated dogs. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.A023