Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: H011
DOI: https://doi.org/10.24326/ICDSUPL3.H011
Published online: 24 April 2024
ICDSUPL, 3, H011 (2024)
Presence of multidrug-resistant Escherichia coli strains in playgrounds in Lublin
Aleksandra Niziołek1*, Anna Bilokinna1, Szymon Strawa1, Ilona Sadok2, Rafał Łopucki3, Marcin Skowronek3
1 Biotechnology Scientific Club of the John Paul II Catholic University of Lublin, Faculty of Medicine, The John Paul II Catholic University of Lublin, Konstantynow 1I, 20-708 Lublin, Poland
2 Department of Chemistry, Institute of Biological Sciences, Faculty of Medicine, The John Paul II Catholic University of Lublin, Konstantynow 1J, 20-708 Lublin, Poland
3 Department of Biomedicine and Environmental Research, Institute of Biological Sciences, Faculty of Medicine, The John Paul II Catholic University of Lublin, Konstantynow 1J, 20-708 Lublin, Poland
* Corresponding author: olaniziolek2002@gmail.com
Abstract
Antimicrobial resistance is a major global public health concern. Nowadays, the resistance of some pathogenic bacteria to traditional treatments is increasing. Surveys conducted in different countries from all over the World have indicated that the prevalence of antibiotic resistance bacteria in playgrounds is worrying. Children represent the principal group at risk for acquiring the infection because of their behavior in playgrounds and immunocompromisation. In this work, we monitored public playgrounds located in Lublin (Poland) in terms of contamination with multidrug-resistant Escherichia coli. Materials and methods: At least 10 different samples (soil, sand, swabs from equipment) from 5 randomly selected public playgrounds were collected. Bacteria were cultivated on various media. After incubation for 48-72 h, single colonies were identified using the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry technique (MALDI-TOF MS) and Biotyper Bruker software. Strains identified as E. coli with a score ≥2.0 were subjected to antibiotic susceptibility testing. To this, paper discs containing one antibiotic have been placed on the Mueller-Hinton agar plate growing bacteria. The following antibiotics were tested: ampicillin (AMP10), amoxicillin + clavulanic acid (AMC30), cefotaxime (CTX30), ceftolozane + tazobactam (CLT40), meropenem (MEM10), imipenem (IPM10), gentamicin (CN10), kanamycin (K30), ciprofloxacin (CIP5), streptomycin (S10), trimethoprim + sulfamethoxazole (SXT25), chloramphenicol (C30), tetracycline (TE30), trimethoprim (W5), sulfonamides (S3 300). CLSI standards were applied for the accepted limits for the diameters of inhibition.
Keywords: playgrounds, Escherichia coli, bacteria, antibiotic resistance, MALDI-TOF MS
How to cite
A. Niziołek, A. Bilokinna, S. Strawa, I. Sadok, R. Łopucki, M. Skowronek, 2024. Presence of multidrug-resistant Escherichia coli strains in playgrounds in Lublin. In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.H011