Volume: 3, 2024
3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: T001
DOI: https://doi.org/10.24326/ICDSUPL3.T001
Published online: 24 April 2024
ICDSUPL, 3, T001 (2024)
The influence of water extraction parameters in subcritical conditions and the shape of the reactor on the quality of extracts obtained from Norway maple (Acer platanoides L.)
Piotr Kamiński1, 2*, Marcin Gruba2, Zygmunt Fekner2, Katarzyna Tyśkiewicz2, Zbigniew Kobus1
1 Department of Technology Fundamentals, University of Life Sciences, Faculty of Production Engineering, Głęboka 28, 20-612 Lublin, Poland
2 Łukasiewicz Research Network — New Chemical Syntheses Institute, Tysiąclecia Państwa Polskiego 13a, 24-110 Puławy, Poland
* Corresponding author: piotr.kaminski@ins.lukasiewicz.gov.pl
Abstract
Water extraction under subcritical conditions is considered an environmentally friendly separation technique for bioactive compounds from plant materials. These techniques can be scaled to industrial size. The bark of trees is a plant material, processed mainly into thermal energy and chemical by-products; it is also a source of valuable biologically active substances. Applying the Box–Behnken methodology, the present research examined the effects of the subcritical water extraction parameters of temperature, process duration, and extractor shape on the amount of total polyphenols content, flavonoids content, and antioxidant activity of bark extracts from Norway maple (Acer platanoides L.). Spectrophotometric (UV-Vis) techniques were employed to evaluate the total polyphenols and flavonoids content. The DPPH radical scavenging method was used to evaluate the antioxidant activity of the extracts. The yield of the process was evaluated through the utilization of response surface methodology (RSM). The temperature of the process has a significant impact on the total amount of polyphenols and flavonoids as well as the antioxidant activity of the obtained extracts. The influence of changes in the process duration and the shape of the pressure cell was not observed. A rise in temperature from 110°C to 170°C caused an 8.9-fold increase in polyphenols content, a 7.2-fold increase in flavonoids content, and a 12.6-fold increase in the antioxidant activity. The maximum values of polyphenols, flavonoids content, and antioxidant properties were reached at a process temperature of 170°C, which is the highest point of the temperature variability range observed in these investigations. The study’s findings demonstrate how crucial it is to select subcritical water extraction variables carefully to achieve the highest extract quality. The temperature rise of the water extraction process in subcritical conditions of Norway maple bark up to 170°C does not result in a decrease in polyphenols and flavonoids content or antioxidant activity. It is suggested that future tests should be performed at higher temperatures, although this may be problematic due to thermal degradation of the raw material. The thermal degradation of extracts was noticeable in previous studies performed on Juglans regia L. bark, where a decrease was observed in antioxidant properties, polyphenols, and flavonoid content for temperatures higher than 131.6°C.
Keywords: maple, Acer platanoides L., subcritical water, Box–Behnken, extraction, polyphenols, flavonoids
How to cite
P. Kamiński, M. Gruba, Z. Fekner, K. Tyśkiewicz, Z. Kobus, 2024. The influence of water extraction parameters in subcritical conditions and the shape of the reactor on the quality of extracts obtained from Norway maple (Acer platanoides L.). In: 3rd International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL3.T001