Volume: 5, 2026
5th International PhD Students’ Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: F004
DOI: https://doi.org/10.24326/ICDSUPL5.F004
Published online: 22 April 2026
Response surface optimization of enzymatic hydrolysis for recovery of anthocyanins and phenolic compounds from strawberry (cv. Malwina) pomace
Greta Grikštaitė-Prapiestė* and Živilė Tarasevičienė
Department of Plant Biology and Food Sciences, Faculty of Agronomy, Agriculture Academy, Vytautas Magnus University, Lithuania
* Corresponding author: greta.grikstaite-prapieste@vdu.lt
The aim of this study was to optimize enzymatic hydrolysis conditions for the efficient recovery of bioactive compounds from strawberry (Fragaria × ananassa cv. Malwina) pomace. A central composite design (CCD) combined with response surface methodology (RSM) was applied to evaluate the effects of hydrolysis time, temperature, and Viscozyme L concentration on total phenolic content (TPC) and total anthocyanin content (TAC).
TPC was quantified using the Folin–Ciocâlteu spectrophotometric method and expressed as milligrams of gallic acid equivalents per milliliter of hydrolysate (mg GAE mL–1), while TAC was determined by the pH differential method and expressed as milligrams of cyanidin-3-glucoside equivalents per milliliter of hydrolysate (mg C3G mL–1).
TAC exhibited the strongest dependence on the process variables and was adequately described by a significant quadratic model (p<0.0001) with high predictive capability (R2=0.9976; predicted R2=0.9383). In contrast, TPC showed a moderate but statistically significant response, best fitted by a linear model, with hydrolysis time identified as the main influencing factor.
The optimal hydrolysis conditions were determined to be 4.03 h, 41–42 °C with approximately 1.0 % (v/w) Viscozyme L. Under these conditions, the predicted values were 1.21 mg GAE mL–1 for TPC and 0.092 mg C3G mL–1 for TAC, yielding an overall desirability of 0.873.
The findings demonstrate that mild enzymatic hydrolysis enables efficient recovery of phenolic compounds and anthocyanins from strawberry pomace while minimizing thermal and enzymatic degradation. This approach supports the valorization of strawberry processing by-products and provides a foundation for further integration with advanced extraction techniques, such as microwave-assisted and ultrasound-assisted extraction.
This study is a part of the science project P-A-25-02 “Extraction of bioactive compounds from strawberry (Fragaria × ananassa Duch) by-products (pomace)” funded by Vytautas Magnus University Science Fund
Keywords: anthocyanins; enzymatic hydrolysis; strawberry by products; valorization
How to cite
Grikštaitė-Prapiestė G., Tarasevičienė Ž., 2026. Response surface optimization of enzymatic hydrolysis for recovery of anthocyanins and phenolic compounds from strawberry (cv. Malwina) pomace. In: 5th International PhD Students’ Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL5.F004