Volume: 5, 2026
5th International PhD Students’ Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A001
DOI: https://doi.org/10.24326/ICDSUPL5.A001
Published online: 22 April 2026
Effect of a standardized allicin dose administered in ovo on hatching results of broiler chickens
Rafał Banaszkiewicz1*, Miłosz Grzejszczak1, Renata Zdun1, Justyna Batkowska2, Karolina Wengerska2 and Kamil Drabik2
1 Students Research Group of Poultry Biology, Breeding, and Husbandry, University of Life Sciences in Lublin, 13 Akademicka St., 20-950 Lublin, Poland
2 Institute of Biological Basis of Animal Production, University of Life Sciences in Lublin, 13 Akademicka St., 20-950 Lublin, Poland
* Corresponding author: rafbana@onet.pl
In ovo injection is one of the techniques for administering active substances to the developing embryo. The injection site and the type of substance administered can influence the final hatching results. The aim of this study was to evaluate the effect of the dose of a standardised allicin solution injected in ovo on the hatching results of broiler chickens.
The research material consisted of 540 hatching eggs from ROSS 308 broiler breeder flocks, divided into 6 experimental groups (each comprising 6 replicate groups of 10 eggs). Before injection, the eggs were weighed, individually labelled, and subjected to standard sanitisation procedures. The eggs were incubated under standard conditions for the species. The injection was performed on the 12th day of incubation into the air chamber using water (positive control) and aqueous solutions of standardised allicin at doses of 100, 300, and 500 µg/ml. In each case, 0.2 ml of the solution was administered. The negative control group consisted of eggs that were not subjected to any manipulation. After incubation was completed, a biological analysis of the hatch was performed, along with an assessment of the quality of the obtained chicks. Regardless of the experimental group, no negative effect of the performed injection on the hatchability of fertilised eggs was observed. Therefore, it can be inferred that allicin administered via this route does not adversely affect embryonic development. Furthermore, differences in body weight and quality were observed among the obtained chicks; however, these observations require further research.
Keywords: broiler breeder eggs; embryonic development; garlic extract; hatchability
How to cite
Banaszkiewicz R., Grzejszczak M., Zdun R., Batkowska J., Wengerska K., Drabik K., 2026. Effect of a standardized allicin dose administered in ovo on hatching results of broiler chickens. In: 5th International PhD Students’ Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL5.A001