Volume: 5, 2026
5th International PhD Students’ Conference at the University of Life Sciences in Lublin, Poland:
ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A035
DOI: https://doi.org/10.24326/ICDSUPL5.A035
Published online: 22 April 2026
Variation within the DAB2IP gene region among pig breeds
Natalia Świerczek* 1, Katarzyna Ropka-Molik 1, Mirosław Tyra 2 and Martyna Małopolska2
1 Department of Animal Molecular Biology, National Research Institute of Animal Production, 1 Krakowska St., 32-083 Balice, Poland
2 Department of Pig Breeding, National Research Institute of Animal Production, 1 Krakowska St., 32-083 Balice, Poland
* Corresponding author: natalia.swierczek@iz.edu.pl
African swine fever is a highly contagious, currently incurable viral disease that leads to mass pig deaths and economic losses. The need to adhere to costly biosecurity policies is changing the structure of the pig market in Europe, limiting the number of small farms and favoring large ones. Current challenges necessitate the search for alternative methods of disease control, including the development of molecular markers linked to increased disease resistance for breeding purposes. Based on the results of comparative WGS performed on pigs that died and survived contact with African swine fever virus (ASFV), a polymorphism within intron 1 of the DAB2IP gene (NC_010443.5:g.261723157G>A) was selected from among many significantly differentiating variants. Its presence was tested in a Polish pig population represented by various breeds. The analysis was performed using Sanger sequencing on 524 individuals of the following breeds: Polish Large White (n=70), Polish Landrace (n=170), Duroc (n=26), Pietrain (n=28), Puławska (n=45), Złotnicka White (n=30), and Złotnicka Spotted (n=155). The analyzed 549 bp fragment contained 18 polymorphic sites, including the studied variant.
Differences in allele and genotype frequencies were observed between breeds. The highest proportion of the g.261723157AA genotype was observed in the Pietrain breed (85.7%), while the highest proportion of the reference genotype g.261723157GG was observed in the Złotnicka Spotted (72.3%). Differences in allele distribution between breeds were statistically significant (p<0.05), excluding the following pairs: Polish Landrace and Large Polish White, Polish Landrace and Duroc, Polish Large Polish White and Duroc, Puławska and Złotnicka White. For the remaining 17 loci identified in the analyzed fragment, differences in variant distribution between populations were also observed. Moreover, the analyzed variant lies within a region annotated as an enhancer, suggesting a potential role in regulating gene expression. However, its functional significance in the context of the response to ASFV infection remains to be further studied. The presented results constitute a preliminary characterization of the genetic variability of the analyzed region in the Polish pig population and can serve as a basis for further association and functional analyses.
Keywords: pig; Sanger sequencing; SNP
How to cite
Świerczek N., Ropka-Molik K., Tyra M., Małopolska M., 2026. Variation within the DAB2IP gene region among pig breeds. In: 5th International PhD Students’ Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL5.A035