Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: E013
DOI: https://doi.org/10.24326/ICDSUPL1.E013
Published online: 26 April 2022
ICDSUPL, 1, E013 (2022)
Potential role of endophytic Methylobacterium strains in heavy metal phytoremediation
Wojciech Sokołowski1*, Sylwia Wdowiak-Wróbel1, Monika Marek-Kozaczuk1, Michał Kalita1, Bożena Kowalczyk1
1 Department of Genetics and Microbiology, Maria Curie Skłodowska University, Akademicka 19, 20-033, Lublin, Poland
* Corresponding author: wojciech.v.sokolowski@gmail.com
Abstract
Phytoremediation is plant-based, cost-effective and environmental-friendly technology of cleanup the pollutants from various ecosystems, therein to reclaim heavy metals from contaminated soils and agrocenosis. In microbial-assisted phytoremediation model, microorganisms with validated abilities to realize various mechanisms useful in remove of metals are success-fully used to enhance the efficiency of this process. Production of metallophores, the secondary metabolites with high affinity to various metals, is the important strategy of heavy metals mobilization and sequestration by plant-associated microorganisms. Eight endophytic, Gram negative, pink-pigmented bacterial isolates, originated from root nodules of various leguminous plants growing in south-eastern Poland and characterized by different genomic DNA fingerprinting profiles (data not shown) were tested. To quantitative evaluation of in vitro ability to produce metallophores, modified chrome azurol S (CAS) agar plate assay was used, where iron ions (Fe) were replaced by the cupper (Cu), aluminum (Al) or arsenic (As). The agar discs (5 mm diameter) cutted from the 7 days old colonies of tested isolates, cultivated on the modified 79CA agar (soil-borne bacteria medium), were placed onto metal-CAS plates in two replicates. The halo zones were measured after 14 days of incubation in 28℃ in dark conditions. The comparative analysis of 16S rRNA gene sequences was used to classify of tested isolates to the genus level. All eight strains of tested bacteria indicate the ability to produce metallophores with affinity to copper (Cu), five strains were able to produce aluminum (Al) affinity compounds and seven strains were able to sequester of arsenic (As). Five strains were able to produce metallophores with affinity to all testes heavy metals. All tested bacteria were classified on the basis of 16S rRNA gene sequences comparative analysis to Methylobacterium genus. Bacteria with the ability to sequestration all tested heavy metals have the potential as phytoremediation-enhancing agents. However, further investigations are required to confirm this conclusion.
How to cite
W. Sokołowski, S. Wdowiak-Wróbel, M. Marek-Kozaczuk, M. Kalita, B. Kowalczyk, 2022. Potential role of endophytic Methylobacterium strains in heavy metal phytoremediation. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/E013