Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A003
DOI: https://doi.org/10.24326/ICDSUPL1.A003
Published online: 26 April 2022
ICDSUPL, 1, A003 (2022)
The effect of a low or high-fat diet and supplementation with various forms of chromium on the biodistribution of this element in the rat’s organism
Aleksandra Marzec1, Zuzanna Całyniuk1*, Radosław Smagieł1, Anna Stępniowska1, Krzysztof Tutaj1, Bartosz Fotschki2, Jerzy Juśkiewicz2, Katarzyna Ognik1
1 Department of Biochemistry and Toxicology, Faculty of Animal Science and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20–950 Lublin, Poland
2 Division of Food Science, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland
* Corresponding author: zcalyniuk@gmail.com
Abstract
The aim of the study was to determine the how the administration of a low-fat or high-fat diet supplemented with various forms of chromium to rats affects accumulation of this element in the tissues and levels of selected mineral elements. The experiment was conducted on 84 male Wistar rats, which were divided into 7 experimental groups. The rats received standard diet (C), low fat diet (LFD) or high fat diet (HFD) with addition of 0.3 mg/kg body weight of chromium (III) picolinate (Cr-Pic) or chromium nanoparticles (Cr-NP). Compared to the control diet, the Cr content of the low-fat diet was 34% lower. The addition of Cr both in the form of Cr-Pic and Cr-NP to the low-fat diet increased the Cr content in this diet by 230% and at the same time increased the Cr intake by rats by 228% compared to the rats receiving the low-fat diet without the addition of Cr. In the case of a low-fat diet with Cr-Pic, the excretion of Cr with urine and faeces increased by 63%, and with Cr-NP by 318% compared to rats receiving a low-fat diet without the addition of Cr. Interestingly, in comparison to rats fed a low-fat diet, rats fed a low-fat diet with the addition of Cr-Pic showed an increase in % retention by 51% and % digestibility of this element in the body by 41%, while with the addition of Cr-NP, a decrease in% retention and digestibility by 27%. Compared to the control diet, the Cr content of the high-fat diet was 33% higher. The addition of Cr in both the Cr-Pic and Cr-NP forms to the high-fat diet increased the Cr content of this diet by 105% and at the same time increased the Cr intake by the rats by 97% as compared to the rats receiving the high-fat diet without the addition of Cr. When a high-fat diet with Cr-Pic or Cr-NP was used, the excretion of Cr with urine and feces increased by approx. 210% compared to rats receiving a high-fat diet without Cr-added. Contrary to rats fed a low-fat diet, the addition of Cr, regardless of its form, to a high-fat diet resulted in a reduction in % retention and % digestibility by approx. 25%. Regardless of the low or high-fat diet, as well as the addition of various forms of Cr, this element did not accumulate in the liver, spleen, kidneys, bones and muscles. It has been established that the fat content of rats’ diets has an influence on the% retention and digestibility of Cr. The retention and digestibility of Cr in rats is lower with a low-fat diet than with a high-fat diet.
This work was supported by the National Science Centre, Grant No. 2020/39/B/NZ9/00674.
How to cite
A. Marzec, Z. Całyniuk, R. Smagieł, A. Stępniowska, K. Tutaj, B. Fotschki, J. Juśkiewicz, K. Ognik, 2022. The effect of a low or high-fat diet and supplementation with various forms of chromium on the biodistribution of this element in the rat’s organism. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/A003