Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A026
DOI: https://doi.org/10.24326/ICDSUPL1.A026
Published online: 26 April 2022
ICDSUPL, 1, A026 (2022)
Effect of the diet containing a coccidiostat or early administration of an antibiotic on the level of selected hormones in turkeys
Radosław Smagieł1*, Ewelina Cholewińska1, Anna Stępniowska1, Dariusz Mikulski2, Katarzyna Ognik1, Jan Jankowski2
1 Department of Biochemistry and Toxicology, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland
2 Department of Poultry Science and Apiculture, University of Warmia and Mazury in Olsztyn, Oczapowskiego 5, 10-719 Olsztyn, Poland
* Corresponding author: radoslaw.smagiel@up.lublin.pl
Abstract
The experiment verified the hypothesis that early administration of the antibiotic to turkeys may adversely increase the level of the stress hormone and adversely affect the secretion of other hormones. The experiment was carried out on 3080 one-day-old turkeys divided into 8 experimental groups. The experiment was carried out in a 2 × 4 factorial design, which included 2 groups of birds (vaccinated, unvaccinated; +, -) and in each 4 treatments (C, M, E, D). Control group (C) did not receive any coccidiostat or antibiotic supplement. Treatment M received coccidiostat monensin at a dose of 90 mg/kg feed, treatment E received enrofloxacin in drinking water for the first 5 days of life (10 mg/kg BW), and treatment D received doxycycline (50 mg/kg BW). The turkeys from the four experimental groups were vaccinated against aMPV (Avian metapnumoviruses) and NDV (Newcastle Disease Virus) on 1st day of life and ORT (Ornithobacterium rhinotracheale) on 28th day of life. On 1st, 7th and 56th day of life, blood was collected from 14 birds from each group for the determination of selected hormones (cortisol, serotonin, thyroxine (T4), histamine, dopamine, noradrenaline). The results were statistically analyzed by two-way ANOVA using the STATISTICA version 13 software. Compared to turkeys fed monensin, 7-day-old turkeys given doxycycline had increased levels of cortisol (P = 0.02) and T4 (P = 0.001). There was no effect of feeding turkeys with fodder with monensin or early administration of enrofloxacin or doxycycline on the blood cortisol level of 56-day-old turkeys, but a decrease in serotonin levels (P < 0.001) was found as a result of the administration of doxycycline. In the blood of 56-day-old turkeys fed with monensin, the level of noradrenaline was higher than in the control group (P = 0.019). Vaccination of the turkeys increased the levels of T4 (P = 0.018) in the blood of 7-day-old turkeys and cortisol (P = 0.009) in the blood of 56-day-old turkeys. Compared to unvaccinated turkeys, vaccination resulted in decreased levels of serotonin (P < 0.001) and noradrenaline (P = 0.01), but only at 56th day of age. Our research confirmed the hypothesis that, as a result of early administration of doxycycline, it is possible to increase the level of cortisol, which is a stress hormone, and to adversely increase the T4 hormone and decrease the level of serotonin. Vaccinating turkeys may exacerbate the reported adverse changes in these hormone levels.
How to cite
R. Smagieł, E. Cholewińska, A. Stępniowska, D. Mikulski, K. Ognik, J. Jankowski, 2022. Effect of the diet containing a coccidiostat or early administration of an antibiotic on the level of selected hormones in turkeys. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/A026