Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: T017
DOI: https://doi.org/10.24326/ICDSUPL1.T017
Published online: 26 April 2022
ICDSUPL, 1, T017 (2022)
Modified screen-printed sensor for determination of anticancer drug candidateuring chemotherapy – case study
Jędrzej Kozak1*, Katarzyna Tyszczuk-Rotko1, Damian Gorylewski1
1 Department of Analytical Chemistry, Institute of Chemical Sciences, Faculty of Chemistry, Maria Curie-Skłodowska University; Pl. Marii Curie-Skłodowskiej 2, 20-031, Lublin, Poland
* Correspondence author: jedrekkozak@onet.pl
Abstract
8-(4-chlorophenyl)-3-phenyl-7,8-dihydroimidazo[2,1-c][1,2,4]triazin-4(6H)-one (4-Cl-PIMT) has been proposed as a new cancer drug candidate. This innovative nucleobase-like molecule revealed significant, concentration dependent antiproliferative effects in human peripheral blood myeloma cells, indicating a possible usefulness in the treatment of such haematological malignancies. Furthermore, this compound proved to have the strongest antimigratory property in human tumour cells of the cervix among all disubstituted fused triazinones, suggesting the highest antimetastatic potential and, consequently, an applicability in the prevention of metastasis. It is worth noting that 4-Cl-PIMT, as a promising candidate for an anticancer agent, was found to be completely nontoxic to normal human skin fibroblast. This presentation shows the results described in published publication (International Journal of Molecular Sciences 23 (2022) 2429-2442). In the article, the first analytical method was proposed for sensitive and selective determination of a novel promising anticancer agent candidate (4-Cl-PIMT) using the carbon nanofibers modified screen-printed carbon sensor (SPCE/CNFs). The SPCE/CNFs sensor coupled with flow system was used for the quantitative determination of 4-Cl-PIMT. The modified sensor showed great improvement to the 4-Cl-PIMT reduction electrode process compared to the unmodified sensor. The reason for enhanced detection performance at the SPCE/CNFs is related to an increase in the number of active sites. To establish the utility of the developed flow voltammetric procedure in biological samples, 4-Cl-PIMT was determined in the spiked human serum samples. The results show satisfactory agreement with the cooperative UHPLC-ESI-MS method.ute to the fight against cancer.
How to cite
J. Kozak, K. Tyszczuk-Rotko, D. Gorylewski, 2022. Modified screen-printed sensor for determination of anticancer drug candidateuring chemotherapy – case study. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/T017