Volume: 1, 2022
1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: ENVIRONMENT – PLANT – ANIMAL – PRODUCT
Abstract number: A034
DOI: https://doi.org/10.24326/ICDSUPL1.A034
Published online: 26 April 2022
ICDSUPL, 1, A034 (2022)
Biopolymer-based gentamicin-enriched biomaterial as a wound dressing
Michał Wójcik1*, Anna Witczyńska2, Anna Wilczyńska3, Agata Przekora1
1 Independent Unit of Tissue Engineering and Regenerative Medicine, Chair of Biomedical Sciences, Faculty of Biomedicine, Medical University of Lublin, Witolda Chodźki 1, 20-093, Lublin, Poland
2 Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland
3 Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Głęboka 30, 20-612 Lublin, Poland
* Corresponding author: michal.wojcik@umlub.pl
Abstract
The skin has many functions in the body, one of them is protection role, when the continuity of its structure is damaged, a wound is created. Specialized and bioactive biomaterials are used to properly and efficiently repair the damaged section of skin. Modern wound dressings are produced using polymers, including those of natural origin, enjoy great interest. They are chosen for their biocompatibility, adequate absorption capacity, stimulation of skin repair and eco-friendly properties. The aim of this work was to produce a biomaterial composed of natural polymers: curdlan and agarose, which could be used as a wound dressing for exudative wounds. Incorporating an aminoglycoside antibiotic into the structure of the biomaterial would additionally allow it to be applied to infected wounds. The biomaterial was created by high-temperature chemical synthesis in an aqueous medium combined with a freeze-drying process. A final obtained foam-like material was used for further tests. The microstructure of the fabricated biomaterial was evaluated by stereoscopic microscopy and SEM technique. To evaluate the gas permeability, the biomaterial was subjected to WVTR test. The ability of the biomaterial to absorb exudate was performed using physiological fluids. The biomaterial was also subjected to biological analyses using normal human skin fibroblasts obtained from ATTC performing the MTT cytotoxicity assay according to ISO 10993-5. The dressing was also evaluated for bactericidal activity against Gram-positive and Gram-negative bacteria. Based on the obtained results it was decided to use the biomaterial as a dressing for a highly infected wound in a veterinary patient. In conclusion, it was proved that the resulting biomaterial exhibits high macroporosity and provides adequate gas exchange. The dressing is able to absorb significant amounts of physiological fluids within its structure, while transforming into a super-absorbent hydrocolloid. The biomaterial has been shown to be non-toxic to skin cells and simultaneously limits the growth of harmful bacteria commonly occurring in infected wounds. Successful alternative treatment for a veterinary patient also proved that the biomaterial could find application as a dressing for highly infected wounds.
The study was supported by NCN in Poland within OPUS 16 grant no. UMO-2018/31/B/ST8/00945. The research was partially supported by the DS3/2021 project within the statutory activity of MUL and Innovation Incubator 4.0 project.
How to cite
M. Wójcik, A. Witczyńska, A. Wilczyńska, A. Przekora, 2022. Biopolymer-based gentamicin-enriched biomaterial as a wound dressing. In: 1st International PhD Student’s Conference at the University of Life Sciences in Lublin, Poland: Environment – Plant – Animal – Product. https://doi.org/10.24326/ICDSUPL1/A034